Isolation and Purification of Active Proteases from Sheep Abomasum and Their Biological Activity

被引：0
|
作者：
B. Liu
O. Abdullaev
O. S. Charyshnikova
Sh. Ya. Mirzaakhmedov
A. Yili
H. A. Aisa
机构：
[1] Chinese Academy of Sciences,Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry
[2] Chinese Academy of Sciences,State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry
[3] University of Chinese Academy of Science,undefined
[4] Educational and Experimental Center for High Technologies,undefined
来源：
Chemistry of Natural Compounds | 2018年 / 54卷
关键词：
sheep abomasum (SA); peptide extraction and isolation; electrophoresis; liquid-chromatography–massspectrometry analysis; sheep abomasum active proteases (SAPs); proteolytic activity; antioxidant activity; lipid peroxidation (LPO); malondialdehyde (MDA);
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Sheep abomasum active proteases (SAPs) were purified by chromatography over a column of Sephadex G-25 and by ultrafiltration. The SAPs were found to contain proteases of molecular mass 14 kDa and active peptides with molecular masses varying in the range 651.39–4248.08 Da. Peptide fractions of SAPs reduced significantly (up to 72.0 ± 2.3% inhibition) accumulation of malondialdehyde (MDA) in brain homogenate of laboratory rats.
引用
收藏
页码：523 / 526
页数：3
相关论文
共 50 条
  • [1] Isolation and Purification of Active Proteases from Sheep Abomasum and Their Biological Activity
    Liu, B.
    Abdullaev, O.
    Charyshnikova, O. S.
    Mirzaakhmedov, Sh. Ya.
    Yili, A.
    Aisa, H. A.
    CHEMISTRY OF NATURAL COMPOUNDS, 2018, 54 (03) : 523 - 526
  • [2] ISOLATION, PURIFICATION AND PROPERTIES OF 2 PROTEASES FROM STREPTOMYCES
    BELLOC, A
    PALLA, JC
    VERRIER, J
    ANNALES DE TECHNOLOGIE AGRICOLE, 1974, 23 (02): : 205 - 214
  • [3] Chemical Composition and Biological Activity of Oil from Lamb Abomasum
    A. Arken
    X. Zhao
    Y. Gao
    A. Wali
    N. Nuermaimaiti
    U. Zh. Ishimov
    Sh.Ya. Mirzaakhmedov
    A. Yili
    Chemistry of Natural Compounds, 2023, 59 : 11 - 14
  • [4] Chemical Composition and Biological Activity of Oil from Lamb Abomasum
    Arken, A.
    Zhao, X.
    Gao, Y.
    Wali, A.
    Nuermaimaiti, N.
    Ishimov, U. Zh.
    Mirzaakhmedov, Sh. Ya.
    Yili, A.
    CHEMISTRY OF NATURAL COMPOUNDS, 2023, 59 (01) : 11 - 14
  • [5] Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates
    Liu, Bing
    Aisa, Haji Akber
    Yili, Abulimiti
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2018, 244 (09) : 1615 - 1625
  • [6] Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates
    Bing Liu
    Haji Akber Aisa
    Abulimiti Yili
    European Food Research and Technology, 2018, 244 : 1615 - 1625
  • [7] THE PASSAGE OF DIGESTA FROM THE ABOMASUM OF SHEEP
    PHILLIPSON, AT
    JOURNAL OF PHYSIOLOGY-LONDON, 1952, 116 (01): : 84 - 97
  • [8] Isolation, purification and biological activity of major docetaxel metabolites from human feces
    Sparreboom, A
    VanTellingen, O
    Scherrenburg, EJ
    Boesen, JJB
    Huizing, MT
    Nooijen, WJ
    Versluis, C
    Beijnen, JH
    DRUG METABOLISM AND DISPOSITION, 1996, 24 (06) : 655 - 658
  • [9] ISOLATION, PURIFICATION, AND BIOLOGICAL-ACTIVITY OF AN INHIBITOR FROM SEPTORIA-TRITICI
    ZELIKOVITCH, N
    EYAL, Z
    KASHMAN, Y
    PHYTOPATHOLOGY, 1992, 82 (03) : 275 - 278
  • [10] ISOLATION, STRUCTURE AND BIOLOGICAL-ACTIVITY OF 2 CHOLECYSTOKININ OCTAPEPTIDES FROM SHEEP BRAIN
    DOCKRAY, GJ
    GREGORY, RA
    HUTCHISON, JB
    HARRIS, JI
    RUNSWICK, MJ
    NATURE, 1978, 274 (5672) : 711 - 713
12345