Isolation and Purification of Active Proteases from Sheep Abomasum and Their Biological Activity

被引：0
|
作者：
B. Liu
O. Abdullaev
O. S. Charyshnikova
Sh. Ya. Mirzaakhmedov
A. Yili
H. A. Aisa
机构：
[1] Chinese Academy of Sciences,Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry
[2] Chinese Academy of Sciences,State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry
[3] University of Chinese Academy of Science,undefined
[4] Educational and Experimental Center for High Technologies,undefined
来源：
Chemistry of Natural Compounds | 2018年 / 54卷
关键词：
sheep abomasum (SA); peptide extraction and isolation; electrophoresis; liquid-chromatography–massspectrometry analysis; sheep abomasum active proteases (SAPs); proteolytic activity; antioxidant activity; lipid peroxidation (LPO); malondialdehyde (MDA);
D O I：
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中图分类号：
学科分类号：
摘要：
Sheep abomasum active proteases (SAPs) were purified by chromatography over a column of Sephadex G-25 and by ultrafiltration. The SAPs were found to contain proteases of molecular mass 14 kDa and active peptides with molecular masses varying in the range 651.39–4248.08 Da. Peptide fractions of SAPs reduced significantly (up to 72.0 ± 2.3% inhibition) accumulation of malondialdehyde (MDA) in brain homogenate of laboratory rats.
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页码：523 / 526
页数：3
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