Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)

被引：9

作者：

Wang, GH ^{[1
]}

Barton, C ^{[1
]}

Rodgers, FG ^{[1
]}

机构：

[1] Natl Lab Enter Pathogens, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 51卷 / 01期

关键词：

bacterial DNA decontamination; RT-PCR; manganese sulfate; microvolume digestion; RNA extraction;

D O I：

10.1016/S0167-7012(02)00041-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For RT-PCR, removal of contaminating genomic DNA in RNA samples using manganese sulfate was more effective, than magnesium. DNA contamination was removed in 3 mug of nucleic acid using 10 U of RNase-free DNase I in 10-mul reaction volumes. The digestion procedure was compatible with commercial RNA extraction kits and was suitable for RT-PCR assay. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 121

页数：3

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