Detection of circulating breast cancer cells by reverse transcriptase polymerase chain reaction (RT-PCR)

被引：15
|
作者：
Hu, XC [1 ]
Chow, LWC [1 ]
机构：
[1] Univ Hong Kong, Queen Mary Hosp, Med Ctr, Dept Surg, Pokfulam, Hong Kong, Peoples R China
来源：
EUROPEAN JOURNAL OF SURGICAL ONCOLOGY | 2000年 / 26卷 / 06期
关键词：
breast cancer; RT-PCR; metastasis; tumour markers;
D O I：
10.1053/ejso.2000.0941
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The confounding problem in treatment of breast cancer is the metastasis of breast tumour. Reverse transcriptase polymerase chain reaction (RT-PCR) has been recently used in the detection of circulating breast cancer cells. This review reports on the development of this assay as well as its advantages and disadvantages. We feel that cytokeratin 20 and beta -human chorionic gonadotropin (hCG) mRNA are the best markers for the detection of circulating boast cancer cells. We suggest that the multiple RNA marker RT-PCR assay can help to increase both sensitivity and specificity of detection, and that quantitative RT-PCR assay is more effective than the qualitative assay in the detection of circulating breast cancer cells. (C) 2000 Harcourt Publishers Ltd.
引用
收藏
页码：530 / 535
页数：6
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