Quantitation and confirmation of six sulphonamides in meat by liquid chromatography-mass spectrometry with photodiode array detection

Multiresidue analysis of six sulphonamides (SAs) (sulphadiazine, sulphadimidine, sulphamonomethoxine, sulphamethoxazole, sulphadimethoxine, and sulphaquinoxaline) in meat (beef, pork, and chicken) using liquid chromatography (LC)-mass spectrometry (MS) with photodiode array (PDA) detection is presented. The sample preparation is carried out by normal-phase matrix solid-phase dispersion (MSPD) with an ethanol solution. The LC-MS determination is performed using a Mightysil RP-4 GP column and an isocratic mobile phase of 0.3% (v/v) acetic acid solution (pH 3.4, in water)-ethanol (83:17, v/v) with an atmospheric pressure chemical ionization (APCI) MS on positive-ion mode. Average recoveries spiked at 0.05-0.5 ppm for each drug were higher than 90% with relative standard deviations between 1% and 6%. In all the processes, no toxic solvents were used at all. (C) 2005 Elsevier Ltd. All rights reserved.