6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in rat brain during development

被引：15
|
作者：
Goren, N
Manzano, A
Riera, L
Ambrosio, S
Ventura, F
Bartrons, R
机构：
[1] Univ Barcelona, Dept Ciencies Fisiol 2, Unitat Bioquim, E-08907 Hospitalet, Spain
[2] Univ Buenos Aires, CONICET, CEFYBO, Fac Odontol,Catedra Farmacol, Buenos Aires, DF, Argentina
来源：
MOLECULAR BRAIN RESEARCH | 2000年 / 75卷 / 01期
关键词：
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase; brain glycolysis; gene expression;
D O I：
10.1016/S0169-328X(99)00319-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
This study reports the expression of the ubiquitous 6-phosphofructo-2-kinase/ftuctose-2,6-bisphosphatase gene (PFKFB3) (PFK-2/FBPase-2) indifferent stages of rat brain development. Northern blot and RT-PCR analysis demonstrated that ubiquitous PFK-2/FBPase-2 is expressed in rat brain from embryonic to adult life and shows a transient increase 1 day before birth, coincident with the maximum concentration of Fru-2,6-P-2 and PFK-2 activity. The levels of brain PFK-2/FBPase-2 gene expression as well as the enzymatic activity and the concentration of Fru-2,6-P-2 appear to be remarkably constant during adult life, without significant differences in the brain hippocampus, cortex, cerebellum or striatum areas. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：138 / 142
页数：5
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