6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in rat brain during development

This study reports the expression of the ubiquitous 6-phosphofructo-2-kinase/ftuctose-2,6-bisphosphatase gene (PFKFB3) (PFK-2/FBPase-2) indifferent stages of rat brain development. Northern blot and RT-PCR analysis demonstrated that ubiquitous PFK-2/FBPase-2 is expressed in rat brain from embryonic to adult life and shows a transient increase 1 day before birth, coincident with the maximum concentration of Fru-2,6-P-2 and PFK-2 activity. The levels of brain PFK-2/FBPase-2 gene expression as well as the enzymatic activity and the concentration of Fru-2,6-P-2 appear to be remarkably constant during adult life, without significant differences in the brain hippocampus, cortex, cerebellum or striatum areas. (C) 2000 Elsevier Science B.V. All rights reserved.