Crystallization and preliminary X-ray diffraction analysis of a novel β-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum

被引：3
|
作者：
Zhu, Zhen [1 ]
He, Miao [1 ]
Huang, Chun-Hsiang [2 ]
Ko, Tzu-Ping [3 ]
Zeng, Yi-Fang [4 ]
Huang, Yu-Ning [4 ]
Jia, Shiru [1 ]
Lu, Fuping [1 ]
Liu, Je-Ruei [4 ,5 ]
Guo, Rey-Ting [2 ]
机构：
[1] Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China
[2] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China
[3] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan
[4] Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan
[5] Natl Taiwan Univ, Dept Anim Sci & Technol, Taipei 106, Taiwan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2014年 / 70卷
基金：
中国国家自然科学基金;
关键词：
EXTENSIN;
D O I：
10.1107/S2053230X14001812
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The beta-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum JCM 1217 hydrolyzes the beta-1,2-linked arabinofuranose disaccharide to release L-arabinoses. HypBA1 was classified into glycoside hydrolase family 127 (GH127) by the CAZy website (http://www.cazy.org/). The enzyme was expressed in Escherichia coli and the purified recombinant protein was crystallized. Crystals belonging to the primitive hexagonal space group P3(x)21, with unit-cell parameters a = b = 75.9, c = 254.0 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.78 angstrom resolution. A BLASTP search (http://blast.ncbi.nlm.nih.gov/) of the Protein Data Bank did not reveal any similar crystal structures. Structural determination by using SeMet MAD and MIR methods is in progress.
引用
收藏
页码：636 / 638
页数：3
相关论文
共 50 条
  • [11] Crystallization and preliminary X-ray diffraction analysis of a novel pectate lyase from Azospirillum irakense
    de Armas, HN
    Rabijns, A
    Verboven, C
    Desair, J
    Vande Broek, A
    Vanderleyden, J
    De Ranter, C
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 292 - 295
  • [12] RETRACTED: Characterization of a Novel β-L-Arabinofuranosidase in Bifidobacterium longum FUNCTIONAL ELUCIDATION OF A DUF1680 FAMILY MEMBER (Retracted Article)
    Fujita, Kiyotaka
    Takashi, Yukari
    Obuchi, Eriko
    Kitahara, Kanefumi
    Suganuma, Toshihiko
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (44) : 38079 - 38085
  • [13] Novel 3-O-α-D-Galactosyl-α-L-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum
    Sasaki, Yuki
    Horigome, Ayako
    Odamaki, Toshitaka
    Xiao, Jin-Zhong
    Ishiwata, Akihiro
    Ito, Yukishige
    Kitahara, Kanefumi
    Fujita, Kiyotaka
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2021, 87 (10) : 1 - 20
  • [14] Crystallization and preliminary X-ray diffraction analysis of vaccinia virus H1L phosphatase
    Roces, Laura
    Knowles, Phillip P.
    Fox, Gavin
    Juanhuix, Jordi
    Scaplehorn, Nicki
    Way, Michael
    McDonald, Neil Q.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 190 - 192
  • [15] Crystallization and preliminary X-ray diffraction analysis of lectin-1 from Pseudomonas aeruginosa
    Karaveg, K
    Liu, ZJ
    Tempel, W
    Doyle, RJ
    Rose, JP
    Wang, BC
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2003, 59 : 1241 - 1242
  • [16] Crystallization and preliminary X-ray diffraction analysis of rat autotaxin
    Marapakala, Kavitha
    Ajees, A. Abdul
    Qin, Jie
    Sankaran, Banumathi
    Rosen, Barry P.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 1050 - 1052
  • [17] Crystallization and preliminary X-ray diffraction analysis of three mastoparans
    Wang, Feng
    Liu, Xiaoqin
    Li, He
    Lang, Xianjun
    Peng, Hui
    Liu, Shenquan
    Jiang, Tao
    PROTEIN AND PEPTIDE LETTERS, 2006, 13 (06): : 629 - 631
  • [18] Crystallization and preliminary X-ray diffraction analysis of human adenovirus
    Reddy, V. S.
    Natchiar, S. K.
    Gritton, L.
    Mullen, T. -M.
    Stewart, P. L.
    Nemerow, G. R.
    VIROLOGY, 2010, 402 (01) : 209 - 214
  • [19] Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Xanthophyllomyces dendrorhous
    Polo, Aitana
    Linde, Dolores
    Estevez, Marta
    Fernandez-Lobato, Maria
    Sanz-Aparicio, Julia
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 1441 - 1444
  • [20] Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum
    Lehwess-Litzmann, Anja
    Neumann, Piotr
    Golbik, Ralph
    Parthier, Christoph
    Tittmann, Kai
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 584 - 586
12345