Crystallization and preliminary X-ray diffraction analysis of rat autotaxin

被引：15

作者：

Marapakala, Kavitha ^{[1
]}

Ajees, A. Abdul ^{[1
]}

Qin, Jie ^{[1
]}

Sankaran, Banumathi ^{[2
]}

Rosen, Barry P. ^{[1
,2
]}

机构：

[1] Florida Int Univ, Dept Cellular Biol & Pharmacol, Herbert Wertheim Coll Med, Miami, FL 33199 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Ctr Struct Biol, Berkeley, CA 94720 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

基金：

美国国家卫生研究院;

关键词：

autotaxin; (ecto)nucleotide pyrophosphatase; phosphodiesterase; 2; lysophosphatidylcholine;

D O I：

10.1107/S1744309110027661

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 A and belonged to space group P1, with unit-cell parameters a = 53.8, b = 63.3, c = 70.5 A, alpha = 98.8, beta = 106.2, gamma = 99.8 degrees. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.

引用

页码：1050 / 1052

页数：3

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