Crystallization and preliminary X-ray diffraction analysis of rat autotaxin

被引：15
|
作者：
Marapakala, Kavitha [1 ]
Ajees, A. Abdul [1 ]
Qin, Jie [1 ]
Sankaran, Banumathi [2 ]
Rosen, Barry P. [1 ,2 ]
机构：
[1] Florida Int Univ, Dept Cellular Biol & Pharmacol, Herbert Wertheim Coll Med, Miami, FL 33199 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Ctr Struct Biol, Berkeley, CA 94720 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷
基金：
美国国家卫生研究院;
关键词：
autotaxin; (ecto)nucleotide pyrophosphatase; phosphodiesterase; 2; lysophosphatidylcholine;
D O I：
10.1107/S1744309110027661
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 A and belonged to space group P1, with unit-cell parameters a = 53.8, b = 63.3, c = 70.5 A, alpha = 98.8, beta = 106.2, gamma = 99.8 degrees. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.
引用
下载
收藏
页码：1050 / 1052
页数：3
相关论文
共 50 条
  • [41] Crystallization and preliminary X-ray diffraction of Trypanosoma cruzi dUTPase
    Bernier-Villamor, V
    Camacho, A
    Gonzalez-Pacanowska, D
    Cedergren-Zeppezauer, E
    Antson, A
    Wilson, KS
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 528 - 530
  • [42] Cinnamomin: separation, crystallization and preliminary X-ray diffraction study
    T. Wang
    Y.-S. Zou
    D.-W. Zhu
    A. Azzi
    W.-Y. Liu
    S.-X. Lin
    Amino Acids, 2008, 34 : 239 - 243
  • [43] Crystallization and preliminary X-ray diffraction studies of human angiostatin
    Abad, MC
    Geiger, J
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 513 - 514
  • [44] Crystallization and preliminary X-ray diffraction study of riboflavin synthase
    Wawrzak, Z
    Viitanen, PV
    Calabrese, JC
    Jordan, DB
    PROTEIN AND PEPTIDE LETTERS, 2001, 8 (01): : 69 - 73
  • [45] Crystallization and preliminary X-ray diffraction studies of recombinant staphylokinase
    Zhan, CH
    Wan, ZL
    Chang, WR
    Yue, JJ
    Liang, DC
    Tang, QQ
    Gu, YL
    Zhang, XX
    Xu, G
    Zhu, YS
    Song, HY
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 : 564 - 565
  • [46] Crystallization and preliminary X-ray analysis of a γ-lactamase
    Gonsalvez, IS
    Isupov, MN
    Littlechild, JA
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2001, 57 : 284 - 286
  • [47] Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45γ
    Zhang, Wenzheng
    Zhao, Mingzhuo
    Li, Jianhui
    Li, Xuemei
    Zeng, ZongHao
    Rao, Zihe
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 1070 - 1073
  • [48] Crystallization and preliminary X-ray diffraction analysis of the arginine repressor of the hyperthermophile Thermotoga neapolitana
    Massant, J
    Peeters, E
    Charlier, D
    Maes, D
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 26 - 28
  • [49] Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor
    Skalova, Tereza
    Dohnalek, Jan
    Ostergaard, Lars Henrik
    Ostergaard, Peter Rahbek
    Kolenko, Petr
    Duskova, Jarmila
    Hasek, Jindrich
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2007, 63 : 1077 - 1079
  • [50] Crystallization and preliminary X-ray diffraction analysis of human phosphate-binding protein
    Contreras-Martel, C
    Carpentier, P
    Morales, R
    Renault, F
    Chesne-Seck, ML
    Rochu, D
    Masson, P
    Fontecilla-Camps, JC
    Chabrière, E
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 67 - 69
12345