Crystallization and preliminary X-ray diffraction analysis of rat autotaxin

被引：15
|
作者：
Marapakala, Kavitha [1 ]
Ajees, A. Abdul [1 ]
Qin, Jie [1 ]
Sankaran, Banumathi [2 ]
Rosen, Barry P. [1 ,2 ]
机构：
[1] Florida Int Univ, Dept Cellular Biol & Pharmacol, Herbert Wertheim Coll Med, Miami, FL 33199 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Ctr Struct Biol, Berkeley, CA 94720 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷
基金：
美国国家卫生研究院;
关键词：
autotaxin; (ecto)nucleotide pyrophosphatase; phosphodiesterase; 2; lysophosphatidylcholine;
D O I：
10.1107/S1744309110027661
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 A and belonged to space group P1, with unit-cell parameters a = 53.8, b = 63.3, c = 70.5 A, alpha = 98.8, beta = 106.2, gamma = 99.8 degrees. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.
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页码：1050 / 1052
页数：3
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