A "turn-on" and proximity ligation assay dependent DNA tweezer for one-step amplified fluorescent detection of DNA

被引：9
|
作者：
Yun, Wen [1 ]
Zhu, Hanhong [1 ]
Wu, Hong [1 ]
Zhuo, Lin [1 ]
Wang, Ruiqi [1 ]
Ha, Xia [1 ]
Wang, Xingmin [1 ]
Zhang, Jiafeng [2 ]
Chen, Hong [3 ]
Yang, Lizhu [2 ]
机构：
[1] Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & New Environm Mat, Chongqing 400067, Peoples R China
[2] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China
[3] Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, 803 Zhongshan North 1st Rd, Shanghai 200083, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 249卷
基金：
上海市自然科学基金;
关键词：
DNA tweezer; Proximity ligation assay; One-step; Human serum; BIOSENSOR; AMPLIFICATION; POLYMERS; MACHINE; WALKING; MOTOR;
D O I：
10.1016/j.saa.2020.119292
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A "turn-on" and proximity ligation assay dependent DNA tweezer was proposed for one-step amplified fluorescent detection of DNA. Target DNA can anneal with capture probe to form an entire long sequence. The formed long sequence can circularly open the hairpin, resulting the "turn-on" of DNA tweezers. A good linear relationship was shown from 40 pM to 20 nM with limit of detection of 10 pM. In addition, it has been successfully utilized to analysis DNA in human serum, representing a great and practical application future. (C) 2020 Elsevier B.V. All rights reserved.
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页数：6
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