A "turn-on" and proximity ligation assay dependent DNA tweezer for one-step amplified fluorescent detection of DNA

被引：9

作者：

Yun, Wen ^{[1
]}

Zhu, Hanhong ^{[1
]}

Wu, Hong ^{[1
]}

Zhuo, Lin ^{[1
]}

Wang, Ruiqi ^{[1
]}

Ha, Xia ^{[1
]}

Wang, Xingmin ^{[1
]}

Zhang, Jiafeng ^{[2
]}

Chen, Hong ^{[3
]}

Yang, Lizhu ^{[2
]}

机构：

[1] Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & New Environm Mat, Chongqing 400067, Peoples R China

[2] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China

[3] Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, 803 Zhongshan North 1st Rd, Shanghai 200083, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 249卷

基金：

上海市自然科学基金;

关键词：

DNA tweezer; Proximity ligation assay; One-step; Human serum; BIOSENSOR; AMPLIFICATION; POLYMERS; MACHINE; WALKING; MOTOR;

D O I：

10.1016/j.saa.2020.119292

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A "turn-on" and proximity ligation assay dependent DNA tweezer was proposed for one-step amplified fluorescent detection of DNA. Target DNA can anneal with capture probe to form an entire long sequence. The formed long sequence can circularly open the hairpin, resulting the "turn-on" of DNA tweezers. A good linear relationship was shown from 40 pM to 20 nM with limit of detection of 10 pM. In addition, it has been successfully utilized to analysis DNA in human serum, representing a great and practical application future. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：6

共 50 条

[31] DNA sequence-dependent fluorescence of doxorubicin for turn-on detection of biothiols in human serum
Xing Chen
Guimei Jiang
Zhili Wang
Shanni Hong
Yuanyuan Zhang
Yahui Guo
Hui Cheng
Jine Wang
Renjun Pei
Analytical and Bioanalytical Chemistry, 2016, 408 : 683 - 693
[32] A CRISPR-Cas12a-based assay for one-step preamplification-free detection of viral DNA
Chen, Yun
Ma, Xiaowei
Pan, Li
Yang, Shuang
Chen, Xiaoying
Wang, Fukai
Yang, Donglei
Li, Min
Wang, Pengfei
SENSORS AND ACTUATORS B-CHEMICAL, 2024, 399
[33] A "turn-on" fluorescent copper biosensor based on DNA cleavage-dependent graphene-quenched DNAzyme
Liu, Meng
Zhao, Huimin
Chen, Shuo
Yu, Hongtao
Zhang, Yaobin
Quan, Xie
BIOSENSORS & BIOELECTRONICS, 2011, 26 (10): : 4111 - 4116
[34] Rapid DNA detection and one-step RNA detection catalyzed by Bst DNA polymerase and narrow-thermal-cycling
Li, Mengzhe
Liu, Mengmeng
Ma, Cuiping
Shi, Chao
ANALYST, 2020, 145 (15) : 5118 - 5122
[35] ONE-STEP PCR METHOD FOR SIMULTANEOUS DETECTION OF HCV RNA AND HBV DNA
HU, KQ
YU, CH
LEE, S
VILLAMIL, FG
VIERLING, JM
HEPATOLOGY, 1993, 18 (04) : A151 - A151
[36] A label-free fluorescent turn-on enzymatic amplification assay for DNA detection using ligand-responsive G-quadruplex formation
Zhao, Chuanqi
Wu, Li
Ren, Jinsong
Qu, Xiaogang
CHEMICAL COMMUNICATIONS, 2011, 47 (19) : 5461 - 5463
[37] Pentaprobe: a comprehensive sequence for the one-step detection of DNA-binding activities
Kwan, AHY
Czolij, R
Mackay, JP
Crossley, M
NUCLEIC ACIDS RESEARCH, 2003, 31 (20)
[38] An amplified single-walled carbon nanotube-mediated chemiluminescence turn-on sensing platform for ultrasensitive DNA detection
Huang, Yong
Zhao, Shulin
Liu, Yi-Ming
Chen, Jia
Chen, Zhen-Feng
Shi, Ming
Liang, Hong
CHEMICAL COMMUNICATIONS, 2012, 48 (75) : 9400 - 9402
[39] Accurate detection of Newcastle disease virus using proximity-dependent DNA aptamer ligation assays
Marnissi, Boutheina
Khalfaoui, Khouloud
Ebai, Tonge
de Oliveira, Felipe Marques Souza
Ghram, Abdeljelil
Kamali-Moghaddam, Masood
Hmila, Issam
FEBS OPEN BIO, 2021, 11 (04): : 1122 - 1131
[40] Iodide etching for one-step quantitative assay of the number of DNA molecules capped on gold nanoparticles
Ding, Yan
Zhang, Yu
Shi, Xiaoqi
Liao, Lingling
Li, Qinghe
Duan, Tingmei
Jiang, Hao
Ding, ZhongXiang
Wang, Hongyan
Liu, Honglin
ANALYTICAL METHODS, 2022, 14 (12) : 1232 - 1238

← 1 2 3 4 5 →