A "turn-on" and proximity ligation assay dependent DNA tweezer for one-step amplified fluorescent detection of DNA

被引：9

作者：

Yun, Wen ^{[1
]}

Zhu, Hanhong ^{[1
]}

Wu, Hong ^{[1
]}

Zhuo, Lin ^{[1
]}

Wang, Ruiqi ^{[1
]}

Ha, Xia ^{[1
]}

Wang, Xingmin ^{[1
]}

Zhang, Jiafeng ^{[2
]}

Chen, Hong ^{[3
]}

Yang, Lizhu ^{[2
]}

机构：

[1] Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & New Environm Mat, Chongqing 400067, Peoples R China

[2] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China

[3] Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, 803 Zhongshan North 1st Rd, Shanghai 200083, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 249卷

基金：

上海市自然科学基金;

关键词：

DNA tweezer; Proximity ligation assay; One-step; Human serum; BIOSENSOR; AMPLIFICATION; POLYMERS; MACHINE; WALKING; MOTOR;

D O I：

10.1016/j.saa.2020.119292

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A "turn-on" and proximity ligation assay dependent DNA tweezer was proposed for one-step amplified fluorescent detection of DNA. Target DNA can anneal with capture probe to form an entire long sequence. The formed long sequence can circularly open the hairpin, resulting the "turn-on" of DNA tweezers. A good linear relationship was shown from 40 pM to 20 nM with limit of detection of 10 pM. In addition, it has been successfully utilized to analysis DNA in human serum, representing a great and practical application future. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：6

共 50 条

[1] A simple and programmed DNA tweezer probes for one-step and amplified detection of UO22+
Xiong, Zhengwei
Wang, Qiang
Zhang, Jiafeng
Yun, Wen
Wang, Xingmin
Ha, Xia
Yang, Lizhu
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2020, 229
[2] Proximity ligation assay induced and DNAzyme powered DNA motor for fluorescent detection of thrombin
Yun, Wen
You, Linfeng
Li, Fukun
Wu, Hong
Chen, Lin
Yang, Lizhu
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2019, 207 : 39 - 45
[3] One-step microchip for DNA fluorescent labeling
Jang, Yeongseok
Shin, Hojun
Jung, Jinmu
Oh, Jonghyun
BIOMEDICAL MICRODEVICES, 2020, 22 (01)
[4] One-step microchip for DNA fluorescent labeling
Yeongseok Jang
Hojun Shin
Jinmu Jung
Jonghyun Oh
Biomedical Microdevices, 2020, 22
[5] One-Step Homogeneous DNA Assay with Single-Nanoparticle Detection
Han, Guojun
Xing, Zhi
Dong, Yanhua
Zhang, Sichun
Zhang, Xinrong
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (15) : 3462 - 3465
[6] Novel styryldehydropyridocolinium derivative as turn-on fluorescent probe for DNA detection
Chang, Lifang
Liu, Chang
He, Song
Lu, Yan
Zhang, Siwen
Zhao, Liancheng
Zeng, Xianshun
SENSORS AND ACTUATORS B-CHEMICAL, 2014, 202 : 483 - 488
[7] Proximity recognition and polymerase-powered DNA walker for one-step and amplified electrochemical protein analysis
Chen, Zhiqiang
Wang, Chao
Hao, Lijie
Gao, Rui
Li, Fang
Liu, Shufeng
BIOSENSORS & BIOELECTRONICS, 2019, 128 : 104 - 112
[8] One-Step Detection of BRCA2 by Fluorescence Turn-On Sensor
Ren Lin-jiao
Wei Ming-hang
Chen Qing-hua
Zhang Pei
Yan Yan-xia
Xue Meng-xiao
Qin Zi-rui
Jiang Li-ying
SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44 (06) : 1526 - 1531
[9] Simple and programmed three-dimensional DNA tweezer for simultaneous one-step detection of ochratoxin A and zearalenone
Chen, Ruipeng
Mao, Zefeng
Lu, Ran
Wang, Zhiguang
Hou, Yue
Zhu, Wenyan
Li, Shuang
Ren, Shuyue
Han, Dianpeng
Liang, Jun
Gao, Zhixian
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 272
[10] Ratiometric electrochemical proximity assay for sensitive one-step protein detection
Ren, Kewei
Wu, Jie
Yan, Feng
Ju, Huangxian
SCIENTIFIC REPORTS, 2014, 4

← 1 2 3 4 5 →