Microfluidic Flow Cytometer for Multiparameter Screening of Fluorophore Photophysics

被引:0
|
作者
Dean, Kevin M. [1 ,2 ]
Davis, Lloyd M. [3 ,4 ]
Lubbeck, Jennifer L. [5 ,6 ,7 ]
Manna, Premashis [5 ,6 ,7 ]
Palmer, Amy E. [1 ,2 ]
Jimenez, Ralph [5 ,6 ,7 ]
机构
[1] Univ Colorado, BioFrontiers Inst, Boulder, CO 80309 USA
[2] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
[3] Univ Tennessee, Inst Space, Dept Phys, Tullahoma, TN 37388 USA
[4] Univ Tennessee, Inst Space, Ctr Laser Applicat, Tullahoma, TN 37388 USA
[5] NIST, JILA, Boulder, CO 80309 USA
[6] NIST, Dept Chem & Biochem, Boulder, CO 80309 USA
[7] Univ Colorado, Boulder, CO 80309 USA
关键词
RED-FLUORESCENT PROTEINS;
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
We present a microfluidic cytometer that sorts mammalian or yeast cells by laser force deflection following real-time multibeam, multiparameter fluorescence measurements, including photobleaching, lifetime and expression level, of the intrinsic fluorophores within each cell.
引用
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页数:2
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