Two-dimensional impedance imaging of cell migration and epithelial stratification

被引：50
|
作者：
Linderholm, Pontus [1 ]
Braschler, Thomas
Vannod, Jeanne
Barrandon, Yann
Brouard, Michel
Renaud, Philippe
机构：
[1] Swiss Fed Inst Technol, Lab Microsyst, CH-1015 Lausanne, Switzerland
[2] Swiss Fed Inst Technol, Lab Stem Cell Dynam, CH-1015 Lausanne, Switzerland
来源：
LAB ON A CHIP | 2006年 / 6卷 / 09期
关键词：
D O I：
10.1039/b603856e
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 mu m x 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min(-1) following a "scratch'' wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.
引用
收藏
页码：1155 / 1162
页数：8
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