Two-dimensional impedance imaging of cell migration and epithelial stratification

被引：50
|
作者：
Linderholm, Pontus [1 ]
Braschler, Thomas
Vannod, Jeanne
Barrandon, Yann
Brouard, Michel
Renaud, Philippe
机构：
[1] Swiss Fed Inst Technol, Lab Microsyst, CH-1015 Lausanne, Switzerland
[2] Swiss Fed Inst Technol, Lab Stem Cell Dynam, CH-1015 Lausanne, Switzerland
来源：
LAB ON A CHIP | 2006年 / 6卷 / 09期
关键词：
D O I：
10.1039/b603856e
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 mu m x 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min(-1) following a "scratch'' wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.
引用
收藏
页码：1155 / 1162
页数：8
相关论文
共 50 条
  • [11] Two-dimensional migration of adsorbed atoms
    Prostnev, A.S.
    Kozhushner, M.A.
    Shub, B.R.
    Chemical Physics Reports, 13 (12):
  • [12] Specific β-containing Integrins Exert Differential Control on Proliferation and Two-dimensional Collective Cell Migration in Mammary Epithelial Cells
    Jeanes, Alexa I.
    Wang, Pengbo
    Moreno-Layseca, Paulina
    Paul, Nikki
    Cheung, Julia
    Tsang, Ricky
    Akhtar, Nasreen
    Foster, Fiona M.
    Brennan, Keith
    Streuli, Charles H.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (29) : 24103 - 24112
  • [13] AC IMPEDANCE MEASUREMENTS OF TWO-DIMENSIONAL SUPERCONDUCTORS
    Jaccard, Y.
    Theron, R.
    Jeanneret, B.
    Meyer, R.
    Leemann, Ch
    Martinoli, P.
    IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY, 1993, 3 (01) : 1640 - 1642
  • [14] THERMODYNAMIC HALL IMPEDANCE FOR TWO-DIMENSIONAL MODELS
    WIDOM, A
    PHYSICAL REVIEW B, 1983, 28 (08): : 4858 - 4859
  • [15] Sensitivity analysis of thorax imaging using two-dimensional Electrical Impedance Tomography (EIT)
    Andiani, L.
    Rubiyanto, A.
    Endarko
    18TH ASIA-OCEANIA CONGRESS OF MEDICAL PHYSICS (AOCMP) & 16TH SOUTH-EAST ASIA CONGRESS OF MEDICAL PHYSICS (SEACOMP), 2019, 1248
  • [16] Two-Dimensional Forward Modeling for Human Thorax Imaging Based on Electrical Impedance Tomography
    Bader, Oumaima
    Hafsa, Mariem
    Ben Amara, Najoua Essoukri
    Kanoun, Olfa
    PROCEEDINGS OF INTERNATIONAL WORKSHOP ON IMPEDANCE SPECTROSCOPY (IWIS 2021), 2021, : 114 - 117
  • [17] Kinetic mechanism of current stratification in two-dimensional structures
    Kachorovskii, VY
    Lyubinskiy, IS
    Tsendin, LD
    10TH INTERNATIONAL SYMPOSIUM ON NANOSTRUCTURES: PHYSICS AND TECHNOLOGY, 2003, 5023 : 282 - 285
  • [18] Two-Dimensional Simulation of Red Blood Cell Deformation and Lateral Migration in Microvessels
    Timothy W. Secomb
    Beata Styp-Rekowska
    Axel R. Pries
    Annals of Biomedical Engineering, 2007, 35 : 755 - 765
  • [19] Two-dimensional simulation of red blood cell deformation and lateral migration in microvessels
    Secomb, Timothy W.
    Styp-Rekowska, Beata
    Pries, Axel R.
    ANNALS OF BIOMEDICAL ENGINEERING, 2007, 35 (05) : 755 - 765
  • [20] TWO-DIMENSIONAL INTERSTITIAL MIGRATION IN THE BCC LATTICE
    JOHNSON, RA
    JOURNAL OF NUCLEAR MATERIALS, 1985, 135 (2-3) : 283 - 285
12345