Whole-Genome Sequencing of the Mycobacterium tuberculosis Manila Sublineage Results in Less Clustering and Better Resolution than Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat (MIRU-VNTR) Typing and Spoligotyping

被引：26
|
作者：
Jamieson, F. B. [1 ,2 ]
Teatero, S. [1 ]
Guthrie, J. L. [1 ]
Neemuchwala, A. [1 ]
Fittipaldi, N. [1 ,2 ]
Mehaffy, C. [1 ,2 ]
机构：
[1] Publ Hlth Ontario, Toronto, ON, Canada
[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 10期
关键词：
NUCLEOTIDE POLYMORPHISMS; EPIDEMIOLOGY; OUTBREAK; STRAINS;
D O I：
10.1128/JCM.01726-14
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mycobacterium tuberculosis isolates of the Manila sublineage are genetically homogeneous. In this study, we used whole-genome sequencing (WGS) to type a collection of 36 M. tuberculosis isolates of the Manila family. WGS enabled the subtyping of these 36 isolates into at least 10 distinct clusters. Our results indicate that WGS is a powerful approach to determining the relatedness of Manila family M. tuberculosis isolates.
引用
收藏
页码：3795 / 3798
页数：4
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