Large-scale de novo DNA synthesis: technologies and applications

被引：20

作者：

Kosuri, Sriram ^{[1
]}

Church, George M. ^{[2
,3
]}

机构：

[1] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

[2] Wyss Inst Biol Inspired Engn, Boston, MA USA

[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA USA

来源：

NATURE METHODS | 2014年 / 11卷 / 05期

关键词：

GENE SYNTHESIS; OLIGONUCLEOTIDE ARRAYS; SYSTEMATIC DISSECTION; INFORMATION-STORAGE; CHEMICAL-SYNTHESIS; PROTEIN-SYNTHESIS; FOLDING DNA; GENOME; POLIOVIRUS; EXPRESSION;

D O I：

10.1038/NMETH.2918

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.

引用

页码：499 / 507

页数：9

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