Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells

被引：28

作者：

Yan, Zhiping ^{[1
]}

Su, Guanyue ^{[1
]}

Gao, Wenbo ^{[1
]}

He, Jia ^{[1
]}

Shen, Yang ^{[1
]}

Zeng, Ye ^{[1
]}

Liu, Xiaoheng ^{[1
]}

机构：

[1] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Inst Biomed Engn, 17,Sect 3,Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China

来源：

CELL ADHESION & MIGRATION | 2019年 / 13卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Fluid shear stress; autophagy; integrin; cytoskeleton; metastasis;

D O I：

10.1080/19336918.2019.1568141

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited the activation of autophagy in HepG2 under FSS. In addition, Cli inhibited the microfilaments formation and expressions of Rac1 and RhoA in HepG2 cells under FSS. Finally, inhibition of autophagy suppressed the cell migration and invasion in HepG2 under FSS. In conclusion, FSS induced autophagy to promote migration and invasion of HepG2 cells via integrin/cytoskeleton pathways.

引用

页码：152 / 162

页数：11

共 50 条

[21] Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
Xv Li
Yajun Wang
Yuzhen Xiong
Jun Wu
Hang Ding
Xiaoyi Chen
Liubo Lan
Haitao Zhang
Scientific Reports, 6
[22] Restoration of miR-20a expression suppresses cell proliferation, migration, and invasion in HepG2 cells
Chen, Guang Shun
Zhou, Ning
Li, Jie-Qun
Li, Ting
Zhang, Zhong-Qiang
Si, Zhong-Zhou
ONCOTARGETS AND THERAPY, 2016, 9 : 3067 - 3076
[23] Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
Li, Xv
Wang, Yajun
Xiong, Yuzhen
Wu, Jun
Ding, Hang
Chen, Xiaoyi
Lan, Liubo
Zhang, Haitao
SCIENTIFIC REPORTS, 2016, 6
[24] Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis
Kuang, Ye
Han, Xiaoyun
Xu, Mu
Yang, Qing
CANCER MEDICINE, 2018, 7 (04): : 1416 - 1429
[25] Trinitrotoluene Induces Endoplasmic Reticulum Stress and Apoptosis in HePG2 Cells
Song, Li
Wang, Yue
Wang, Jun
Yang, Fan
Li, Xiaojun
Wu, Yonghui
MEDICAL SCIENCE MONITOR, 2015, 21
[26] OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells
Ren, Jie
Liu, Yu
Li, Lixia
Zhao, Yuexin
Li, Zhongyu
Wu, Chao
Chen, Lin
Hu, Kun
CELL BIOLOGY INTERNATIONAL, 2018, 42 (02) : 194 - 204
[27] Galangin Induces Autophagy through Upregulation of p53 in HepG2 Cells
Wen, Min
Wu, Jun
Luo, Hui
Zhang, Haitao
PHARMACOLOGY, 2012, 89 (5-6) : 247 - 255
[28] MicroRNA-224 is upregulated in HepG2 cells and involved in cellular migration and invasion
Li, Qiong
Wang, Ge
Shan, Jin-Lu
Yang, Zhi-Xiang
Wang, Hong-Zhong
Feng, Jin
Zhen, Ji-Jun
Chen, Chuan
Zhang, Zhi-Min
Xu, Wen
Luo, Xi-Zhong
Wang, Dong
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2010, 25 (01) : 164 - 171
[29] Autophagic blockage by bismuth sulfide nanoparticles inhibits migration and invasion of HepG2 cells
Hao, Bai-Mei
Liu, Ya-Nan
Zhang, Chen-Yang
Li, Guang-Qi
Wang, Wan-Ni
Xu, Wei-Dong
Zha, Zheng-Bao
Wang, Feng
Li, Chao
Miao, Zhao-Hua
Yang, Xiao-Xiao
Chen, Yuan-Li
Qian, Hai-Sheng
Zhou, Wei
NANOTECHNOLOGY, 2020, 31 (46)
[30] High glucose induces inflammatory responses in HepG2 cells via the oxidative stress-mediated activation of NF-κB, and MAPK pathways in HepG2 cells
Panahi, Ghodratollah
Pasalar, Parvin
Zare, Mina
Rizzuto, Rosario
Meshkani, Reza
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 2018, 124 (05) : 468 - 474

← 1 2 3 4 5 →