Isothermal Amplification Strategies for Detection in Microfluidic Devices

被引：18
|
作者：
Sarkar, Saheli [1 ]
Sabhachandani, Pooja [1 ]
Konry, Tania [1 ]
机构：
[1] Northeastern Univ, Dept Pharmaceut Sci, 360 Huntington Ave, Boston, MA 02115 USA
来源：
TRENDS IN BIOTECHNOLOGY | 2017年 / 35卷 / 03期
关键词：
SINGLE CELLS; CIRCLE AMPLIFICATION; LIGATION ASSAYS; CHIP; PROTEOME;
D O I：
10.1016/j.tibtech.2016.09.006
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for micro fluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.
引用
收藏
页码：186 / 189
页数：4
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