Inhibitory activity of hinokitiol against biofilm formation in fluconazole-resistant Candida species

被引：30
|
作者：
Kim, Dae Jin [1 ]
Lee, Min Woo [1 ]
Choi, Jeong Su [1 ]
Lee, Seung Gwan [1 ]
Park, Jee Yoon [2 ]
Kim, Suhng Wook [1 ]
机构：
[1] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Seoul, South Korea
[2] Sungkyunkwan Univ, Kangbuk Samsung Hosp, Dept Obstet & Gynecol, Sch Med, Seoul, South Korea
来源：
PLOS ONE | 2017年 / 12卷 / 02期
关键词：
ALBICANS BIOFILMS; ESSENTIAL OIL; PLANT-ORIGIN; INFECTIONS; TERPENOIDS; COMPONENTS; LINALOOL; GROWTH;
D O I：
10.1371/journal.pone.0171244
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to investigate the ability of hinokitiol to inhibit the formation of Candida biofilms. Biofilm inhibition was evaluated by quantification of the biofilm metabolic activity with XTT assay. Hinokitiol efficiently prevented biofilm formation in both fluconazole-susceptible and fluconazole-resistant strains of Candida species. We determined the expression levels of specific genes previously implicated in biofilm development of C. albicans cells by realtime RT-PCR. The expression levels of genes associated with adhesion process, HWP1 and ALS3, were downregulated by hinokitiol. Transcript levels of UME6 and HGC1, responsible for long-term hyphal maintenance, were also decreased by hinokitiol. The expression level of CYR1, which encodes the component of signaling pathway of hyphal formation-cAMP-PKA was suppressed by hinokitiol. Its upstream general regulator RAS1 was also suppressed by hinokitiol. These results indicate that hinokitiol may have therapeutic potential in the treatment and prevention of biofilm-associated Candida infections.
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页数：11
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