Synergistic anticandidal activity of etomidate and azoles against clinical fluconazole-resistant Candida isolates

被引：11
|
作者：
Sa, Livia G. do A. V. [1 ,2 ]
da Silva, Cecilia R. [1 ,2 ]
Campos, Rosana de S. [1 ,2 ,3 ]
Neto, Joao B. de A. [1 ,2 ,3 ]
Sampaio, Leticia S. [1 ,2 ]
do Nascimento, Francisca B. S. A. [1 ,2 ]
Barroso, Fatima D. D. [1 ,2 ]
da Silva, Lisandra J. [1 ,2 ]
Queiroz, Helaine A. [1 ,2 ]
Candido, Thiago M. [1 ,2 ,3 ]
Rodrigues, Daniel S. [1 ,2 ]
Leitao, Amanda C. [1 ,2 ]
de Moraes, Manoel O. [2 ]
Cavalcanti, Bruno C. [2 ]
Junior, Helio V. N. [1 ,2 ]
机构：
[1] Univ Fed Ceara, Lab Bioprospect Antimicrobial Mol LABIMAN, Sch Pharm, Dept Clin & Toxicol Anal, BR-60430116 Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Drug Res & Dev Ctr, BR-60430276 Fortaleza, Ceara, Brazil
[3] Univ Ctr Christus, BR-60160230 Fortaleza, Ceara, Brazil
来源：
FUTURE MICROBIOLOGY | 2019年 / 14卷 / 17期
关键词：
azoles; biofilm; Candida spp; etomidate; synergism; ANTIMICROBIAL ACTIVITY; CELL-LINE; ALBICANS; SUSCEPTIBILITY; MECHANISMS; PROPOFOL; RHODAMINE-123; COMBINATION; MANAGEMENT; GUIDELINE;
D O I：
10.2217/fmb-2019-0075
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: The purpose of this study was to evaluate the effect of etomidate alone and in combination with azoles on resistant strains of Candida spp. in both planktonic cells and biofilms. Materials & methods: The antifungal activity of etomidate was assessed by the broth microdilution test; flow cytometric procedures to measure fungal viability, mitochondrial transmembrane potential, free radical generation and cell death; as well detection of DNA damage using the comet assay. The interaction between etomidate and antifungal drugs (itraconazole and fluconazole) was evaluated by the checkerboard assay. Results: Etomidate showed antifungal activity against resistant strains of Candida spp. in planktonic cells and biofilms. Etomidate also presented synergism with fluconazole and itraconazole in planktonic cells and biofilms. Conclusion: Etomidate showed antifungal activity against Candida spp., indicating that it is a possible therapeutic alternative.
引用
收藏
页码：1477 / 1488
页数：12
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