Fiber-optic fluorescence microscopy for functional brain imaging in awake, mobile mice

被引：2
|
作者：
Cha, Jaepyeong [1 ]
Paukert, Martin [2 ,3 ]
Bergles, Dwight E. [2 ]
Kang, Jin U. [1 ]
机构：
[1] Johns Hopkins Univ, Dept Elect & Comp Engn, 3400 N Charles St, Baltimore, MD 21218 USA
[2] Johns Hopkins Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21205 USA
[3] Univ Texas Hlth Sci Ctr San Antonio, Dept Physiol, San Antonio, TX 78229 USA
来源：
OPTICAL TECHNIQUES IN NEUROSURGERY, NEUROPHOTONICS, AND OPTOGENETICS | 2014年 / 8928卷
关键词：
Fiber-optic sensor; Fluorescence microendoscopy; GCaMP3; Functional brain imaging; Astrocyte calcium transients; cerebellum; CELLS;
D O I：
10.1117/12.2038265
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Fiber-optic based optical imaging is an emerging technique for studying brain activity in live animals. Here, we introduce a novel fluorescence fiber-optic microendoscopy approach to minimal invasively detect neural activities in a live mouse brain. The system uses a flexible endoscopic probe composed of a multi-core coherent fiber-bundle terminated with an approximately 1500-micron working distance objective lens. The fiber-optic neural interface is mounted on a 4-mm(2) cranial window enabling visualization of glial calcium transients from the same brain region for weeks. We evaluated the system performance through in vivo imaging of GCaMP3 fluorescence in transgenic head-restrained mice during locomotion.
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页数：6
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