Three-dimensional fiber-optic readout of single-neuron-resolved fluorescence in living brain of transgenic mice

被引：9

作者：

Fedotov, I. V. ^{[1
,2
,3
]}

Pochechuev, M. S. ^{[1
,2
]}

Ivashkina, O. I. ^{[2
]}

Fedotov, A. B. ^{[1
,2
,3
,4
]}

Anokhin, K. V. ^{[2
,5
]}

Zheltikov, A. M. ^{[1
,2
,3
,4
]}

机构：

[1] MV Lomonosov MSU, Phys Dept, Int Laser Ctr, Moscow, Russia

[2] Natl Res Ctr, Kurchatov Inst, Moscow, Russia

[3] Russian Quantum Ctr, Skolkovo, Moscow Region, Russia

[4] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA

[5] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow, Russia

来源：

JOURNAL OF BIOPHOTONICS | 2017年 / 10卷 / 6-7期

关键词：

neurophotonics; fiber-optic neurointerfaces; optogenetics; FREELY MOVING MICE; INTERROGATION; CIRCUITS;

D O I：

10.1002/jbio.201600002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bundle of individually addressable optical fibers is shown to enable three-dimensional optical readout from single neurons in live brain, as well as in intact brain extracted from transgenic mice. With individual fibers in the bundle being only a few microns in diameter, single neurons are readily resolved in brain images transmitted by the fiber bundle. The third dimension is added by scanning the fiber in the longitudinal direction, with the fluorescence return read out from only one of the fibers in the bundle.

引用

页码：775 / 779

页数：5

共 20 条

[1] Fiber-optic fluorescence microscopy for functional brain imaging in awake, mobile mice
Cha, Jaepyeong
Paukert, Martin
Bergles, Dwight E.
Kang, Jin U.
OPTICAL TECHNIQUES IN NEUROSURGERY, NEUROPHOTONICS, AND OPTOGENETICS, 2014, 8928
[2] Three-dimensional printed microcantilever with mechanical metamaterial for fiber-optic microforce sensing
Wang, Famei
Zou, Mengqiang
Liao, Changrui
Li, Bozhe
Liu, Dejun
Zhou, Jie
Huang, Haoqiang
Zhao, Jinlai
Liu, Chao
Chu, Paul K.
Wang, Yiping
APL PHOTONICS, 2023, 8 (09)
[3] A three-dimensional shape measurement system based on fiber-optic image bundles
Zhen, Cheng
Zhao, Huijie
Liang, Xiaoyue
Jiang, Hongzhi
Lv, Peijun
Li, Hong
OPTOELECTRONIC IMAGING AND MULTIMEDIA TECHNOLOGY III, 2014, 9273
[4] Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver
Xia, Wenfeng
West, Simeon J.
Finlay, Malcolm C.
Pratt, Rosalind
Mathews, Sunish
Mari, Jean-Martial
Ourselin, Sebastien
David, Anna L.
Desjardins, Adrien E.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2018, (138):
[5] Three-dimensional shape reconstruction system based on fiber-optic interference fringe imaging
Duan, Xiaojie
Duan, Fajie
Lv, Changrong
INTERNATIONAL JOURNAL OF IMAGING SYSTEMS AND TECHNOLOGY, 2012, 22 (04) : 208 - 213
[6] Three-dimensional shape measurement with sinusoidal phase-modulating fiber-optic interferometer fringe
Lv, Changrong
Duan, Fajie
Fu, Xiao
Huang, Tingting
OPTICAL FIBER TECHNOLOGY, 2016, 29 : 20 - 27
[7] Fault-tolerant variable fiber-optic attenuator using three-dimensional beam spoiling
Riza, NA
Sumriddetchkajorn, S
OPTICS COMMUNICATIONS, 2000, 185 (1-3) : 103 - 108
[8] Three-Dimensional Shape Measurement with Wavelength-Modulated Laser Base on Fiber-Optic Interferometer Projection
Zhu, Ronggang
Song, Qian
Zhu, Rihong
Li, Jianxin
INTERNATIONAL CONFERENCE ON OPTICS IN PRECISION ENGINEERING AND NANOTECHNOLOGY (ICOPEN2013), 2013, 8769
[9] Three-dimensional electromagnetic modeling of fiber-core effects on the coupling characteristics of weakly fused tapered fiber-optic couplers
Wu, TL
JOURNAL OF LIGHTWAVE TECHNOLOGY, 2000, 18 (07) : 1024 - 1030
[10] Three-dimensional waveguide arrays for coupling between fiber-optic connectors and surface-mounted optoelectronic devices
Hiramatsu, S
Kinoshita, M
JOURNAL OF LIGHTWAVE TECHNOLOGY, 2005, 23 (09) : 2733 - 2739

← 1 2 →