Three-dimensional fiber-optic readout of single-neuron-resolved fluorescence in living brain of transgenic mice

被引：9
|
作者：
Fedotov, I. V. [1 ,2 ,3 ]
Pochechuev, M. S. [1 ,2 ]
Ivashkina, O. I. [2 ]
Fedotov, A. B. [1 ,2 ,3 ,4 ]
Anokhin, K. V. [2 ,5 ]
Zheltikov, A. M. [1 ,2 ,3 ,4 ]
机构：
[1] MV Lomonosov MSU, Phys Dept, Int Laser Ctr, Moscow, Russia
[2] Natl Res Ctr, Kurchatov Inst, Moscow, Russia
[3] Russian Quantum Ctr, Skolkovo, Moscow Region, Russia
[4] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA
[5] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow, Russia
来源：
JOURNAL OF BIOPHOTONICS | 2017年 / 10卷 / 6-7期
关键词：
neurophotonics; fiber-optic neurointerfaces; optogenetics; FREELY MOVING MICE; INTERROGATION; CIRCUITS;
D O I：
10.1002/jbio.201600002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A bundle of individually addressable optical fibers is shown to enable three-dimensional optical readout from single neurons in live brain, as well as in intact brain extracted from transgenic mice. With individual fibers in the bundle being only a few microns in diameter, single neurons are readily resolved in brain images transmitted by the fiber bundle. The third dimension is added by scanning the fiber in the longitudinal direction, with the fluorescence return read out from only one of the fibers in the bundle.
引用
收藏
页码：775 / 779
页数：5
相关论文
共 20 条
  • [11] Fiber-Optic Interferometer Projection Based on Spatial-Temporal Fringes for Three-Dimensional Surface Measurement Technique
    Li Haoyu
    Zhu Ronggang
    Yong, He
    LASER & OPTOELECTRONICS PROGRESS, 2018, 55 (10)
  • [12] Fabrication of three-dimensional zinc oxide nanoflowers for high-sensitivity fiber-optic ammonia gas sensors
    Zhu, Yi
    Fu, Haiwei
    Ding, Jijun
    Li, Huidong
    Zhang, Min
    Zhang, Jingle
    Liu, Yinggang
    APPLIED OPTICS, 2018, 57 (27) : 7924 - 7930
  • [13] Numerical modeling of weakly fused fiber-optic polarization beamsplitters - Part II: The three-dimensional electromagnetic model
    Yang, SW
    Wu, TL
    Wu, CW
    Chang, HC
    JOURNAL OF LIGHTWAVE TECHNOLOGY, 1998, 16 (04) : 691 - 696
  • [14] Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells
    Deniset-Besseau, A.
    Leveque-Fort, S.
    Fontaine-Aupart, M. P.
    Roger, G.
    Georges, P.
    APPLIED OPTICS, 2007, 46 (33) : 8045 - 8051
  • [15] Three-Dimensional Topological Reconstruction of the Sensing Coil of a Fiber-Optic Gyroscope Using X-Ray Computed Tomography
    Pillon, Jeremie
    Collignon, Matthieu
    Rattier, Maxime
    Louf, Francois
    Peter, Emmanuelle
    Boucard, Pierre-Alain
    Lefevre, Herve C.
    JOURNAL OF LIGHTWAVE TECHNOLOGY, 2021, 39 (14) : 4861 - 4872
  • [16] Respiratory function monitoring using a real-time three-dimensional fiber-optic shaping sensing scheme based upon fiber Bragg gratings
    Allsop, Thomas
    Bhamber, Ranjeet
    Lloyd, Glynn
    Miller, Martin R.
    Dixon, Andrew
    Webb, David
    Ania Castanon, Juan Diego
    Bennion, Ian
    JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (11)
  • [17] Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes
    Martin, Abraham
    Aguirre, Juan
    Sarasa-Renedo, Ana
    Tsoukatou, Debbie
    Garofalakis, Anikitos
    Meyer, Heiko
    Mamalaki, Clio
    Ripoll, Jorge
    Planas, Anna M.
    MOLECULAR IMAGING, 2008, 7 (04): : 157 - 167
  • [18] Performance of asynchronous fiber-optic code division multiple access system based on three-dimensional wavelength/time/space codes and its link analysis
    Singh, Jaswinder
    APPLIED OPTICS, 2010, 49 (08) : 1355 - 1363
  • [19] Three-Dimensional β-Amyloid Burden Correlation Between the Eye and Brain in Alzheimer's Disease Mice Using Light-Sheet Fluorescence Microscopy
    Son, Hye Joo
    Kim, Seonok
    Kim, Seog-Young
    Jung, Jin Hwa
    Lee, Suk Hyun
    Kim, Soo-Jong
    Kim, Chanwoo
    Hahn, Alice
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2025, 66 (03)
  • [20] Novel three-dimensional analysis tool for vascular trees indicates complete micro-networks, not single capillaries, as the angiogenic endpoint in mice overexpressing human VEGF165 in the brain
    Heinzer, Stefan
    Kuhn, Gisela
    Krucker, Thomas
    Meyer, Eric
    Ulmann-Schuler, Alexandra
    Stampanoni, Marco
    Gassmann, Max
    Marti, Hugo H.
    Mueller, Ralph
    Vogel, Johannes
    NEUROIMAGE, 2008, 39 (04) : 1549 - 1558
12