Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2

被引：6
|
作者：
Gowdhaman, Dharmalingam [1 ]
Jeyalakshmi, Ganesan [1 ]
Sugumaran, Karuppiah [1 ]
Subramanian, Nagarajan Sai [1 ]
Santhosh, Ramachandran Sarojini [1 ]
Ponnusami, Venkatachalam [1 ]
机构：
[1] SASTRA Univ, Sch Chem & Biotechnol, Thirumalaisamudram 613401, Thanjavur, India
来源：
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | 2014年 / 39卷 / 01期
关键词：
Paper mill effluent; Bacillus aerophilus KGJ2; xylanase; thermophilic; THERMOSTABLE XYLANASE; STABLE XYLANASE;
D O I：
10.5505/tjb.2014.92300
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Objective: The objective of the present study was to isolate a potential and novel Bacillus strain from paper mill effluent for the production of an industrially important xylanase. Material and Methods: A potent xylanase producing microorganism was isolated from paper mill effluent based on zone of clearance on xylan agar medium. The strain was identified based on 16S rRNA analysis and biochemical characterization. Xylanase produced by the isolated strain was partially purified and characterized for its activity and stability. Results: The xylanase produced by this Bacillus aerophilus KGJ2 was thermophilic, shows higher activity and stability at 70 degrees C. Xylanase had activity peak at pH 4.0 and was very acid stable. Birchwood xylan and beef extract were identified as best suited carbon source and nitrogen source, respectively. Conclusion: The results confirm that Bacillus aerophilus KGJ2 produced a unique acidothermotolerant xylanase.
引用
收藏
页码：70 / 77
页数：8
相关论文
共 50 条
  • [1] Xylanase production from Bacillus aerophilus KGJ2 and its application in xylooligosaccharides preparation
    Gowdhaman, D.
    Manaswini, V. S.
    Jayanthi, V.
    Dhanasri, M.
    Jeyalakshmi, G.
    Gunasekar, V.
    Sugumaran, K. R.
    Ponnusami, V.
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2014, 64 : 90 - 98
  • [2] Production and optimization of xylooligosaccharides from corncob by Bacillus aerophilus KGJ2 xylanase and its antioxidant potential
    Gowdhaman, D.
    Ponnusami, V.
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 79 : 595 - 600
  • [3] Optimization of cellulase production by newly isolated Bacillus sp
    Mangaoglu, H.
    Avci, N. G.
    Akbulut, B. Saryyar
    Kazan, D.
    [J]. FEBS JOURNAL, 2010, 277 : 202 - 202
  • [4] Production and Optimization of Xylanase by Thermophilic Bacillus sp Isolated from Soil
    Lawrence, Rubina
    Kumar, Yashab
    Singh, Ajay Kumar
    Singh, Satendra
    [J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2015, 9 (02): : 1117 - 1128
  • [5] PRODUCTION OF ALKALINE XYLANASE BY A NEWLY ISOLATED ALKALIPHILIC BACILLUS SP STRAIN 41M-1
    NAKAMURA, S
    WAKABAYASHI, K
    NAKAI, R
    AONO, R
    HORIKOSHI, K
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1993, 9 (02): : 221 - 224
  • [6] A novel broad-temperature active and solvent stable esterase from a newly isolated Bacillus aerophilus
    Charoenpanich, Jittima
    Soongrung, Tewarit
    Chinnasri, Supattra
    Suebchuea, Naruemon
    Suppoontong, Montira
    Thiemsawait, Suthangrat
    [J]. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2018, 13 : 116 - 122
  • [7] Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19
    Cui, Fengjie
    Li, Yin
    Liu, Zhiqiang
    Zhao, Hui
    Ping, Lifeng
    Ping, Liying
    Yang, Yinan
    Xue, Yaping
    Yan, Lijiao
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2009, 25 (04): : 721 - 725
  • [8] Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19
    Fengjie Cui
    Yin Li
    Zhiqiang Liu
    Hui Zhao
    Lifeng Ping
    Liying Ping
    Yinan Yang
    Yaping Xue
    Lijiao Yan
    [J]. World Journal of Microbiology and Biotechnology, 2009, 25 : 721 - 725
  • [9] Statistical Optimization of Medium Compositions for Alkaline Protease Production by Newly Isolated Bacillus amyloliquefaciens
    Cheng, S. -W.
    Wang, Y. -F.
    Wang, M. -L.
    [J]. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY, 2012, 26 (03) : 225 - 231
  • [10] Xylanase Isozymes from the Newly Isolated Bacillus sp CKBx1D and Optimization of Its Deinking Potentiality
    Maity, Chiranjit
    Ghosh, Kuntal
    Halder, Suman K.
    Jana, Arijit
    Adak, Atanu
    Das Mohapatra, Pradeep K.
    Pati, Bikas R.
    Mondal, Keshab C.
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 167 (05) : 1208 - 1219
12345