Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2

被引：6

作者：

Gowdhaman, Dharmalingam ^{[1
]}

Jeyalakshmi, Ganesan ^{[1
]}

Sugumaran, Karuppiah ^{[1
]}

Subramanian, Nagarajan Sai ^{[1
]}

Santhosh, Ramachandran Sarojini ^{[1
]}

Ponnusami, Venkatachalam ^{[1
]}

机构：

[1] SASTRA Univ, Sch Chem & Biotechnol, Thirumalaisamudram 613401, Thanjavur, India

来源：

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | 2014年 / 39卷 / 01期

关键词：

Paper mill effluent; Bacillus aerophilus KGJ2; xylanase; thermophilic; THERMOSTABLE XYLANASE; STABLE XYLANASE;

D O I：

10.5505/tjb.2014.92300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Objective: The objective of the present study was to isolate a potential and novel Bacillus strain from paper mill effluent for the production of an industrially important xylanase. Material and Methods: A potent xylanase producing microorganism was isolated from paper mill effluent based on zone of clearance on xylan agar medium. The strain was identified based on 16S rRNA analysis and biochemical characterization. Xylanase produced by the isolated strain was partially purified and characterized for its activity and stability. Results: The xylanase produced by this Bacillus aerophilus KGJ2 was thermophilic, shows higher activity and stability at 70 degrees C. Xylanase had activity peak at pH 4.0 and was very acid stable. Birchwood xylan and beef extract were identified as best suited carbon source and nitrogen source, respectively. Conclusion: The results confirm that Bacillus aerophilus KGJ2 produced a unique acidothermotolerant xylanase.

引用

下载

页码：70 / 77

页数：8

共 50 条

[31] Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation
Irfan, Muhammad
Asghar, Umar
Nadeem, Muhammad
Nelofer, Rubina
Syed, Quratulain
JOURNAL OF RADIATION RESEARCH AND APPLIED SCIENCES, 2016, 9 (02) : 139 - 147
[32] Utilization of Agroresidues for the Production of Xylanase by Bacillus safensis XPS7 and Optimization of Production Parameters
Devi, Shikha
Dwivedi, Divya
Bhatt, Arvind Kumar
FERMENTATION-BASEL, 2022, 8 (05):
[33] Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5
Jalal, Amir
Rashid, Naeem
Rasool, Nouman
Akhtar, Muhammad
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 107 (04) : 360 - 365
[34] Xylanase production by a newly isolated Aspergillus niger SS7 in submerged culture
Bakri, Yasser
Al-Jazairi, Manal
Al-Kayat, Ghassan
POLISH JOURNAL OF MICROBIOLOGY, 2008, 57 (03) : 249 - 251
[35] Production of xylanase from a newly isolated Penicillium sp. ZH-30
Yin Li
Zhiqiang Liu
Fengjie Cui
Yingying Xu
Hui Zhao
World Journal of Microbiology and Biotechnology, 2007, 23 : 837 - 843
[36] Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase
Dash, Biplab Kumar
Rahman, M. Mizanur
Sarker, Palash Kumar
BIOMED RESEARCH INTERNATIONAL, 2015, 2015
[37] Production of Xylanase by a Bacillus sp 2B5 Isolated from Paper Mills in Kahramanmaras, Turkey
Kiran, Ozlem
Ucar, Gulsum
ASIAN JOURNAL OF CHEMISTRY, 2009, 21 (05) : 3489 - 3492
[38] Utilization of corn cob waste for xylanase production by newly isolated Bacillus pumilus XRL5 and its application in saccharification of lignocellulosic biomass
Rana, Nidhi
Sharma, Vaishali
Rathour, Ranju Kumari
Abuja, Vishal
Bhatia, Ravi Kant
Bhatt, Arvind Kumar
TRENDS IN CARBOHYDRATE RESEARCH, 2019, 11 (03) : 20 - 32
[39] Thermostable and alkaline tolerant xylanase production by Bacillus subtilis isolated form marine environment
Annamalai, N.
Thavasi, R.
Jayalakshmi, S.
Balasubramanian, T.
INDIAN JOURNAL OF BIOTECHNOLOGY, 2009, 8 (03): : 291 - 297
[40] Response surface optimization of the critical medium components for the production of alkaline protease by a newly isolated Bacillus sp.
Adinarayana, K
Ellaiah, P
JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 2002, 5 (03): : 281 - 287

← 1 2 3 4 5 →