Detection of enteroviruses from water samples using optimised RT-PCR with universal primers

被引：1
|
作者：
Zhang, C. M. [1 ]
Liu, Y. J. [1 ]
Wang, X. C. [1 ]
Xue, X. P. [2 ]
机构：
[1] Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Peoples R China
[2] Northwestern Polytech Univ, Fac Life Sci, Xian 710072, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENT AND POLLUTION | 2009年 / 38卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
enteroviruses; universal primers; RT-PCR; simultaneous detection; concentration; microporous filter; HEPATITIS-A VIRUS; ENTERIC VIRUSES; INFECTIOUS ENTEROVIRUSES; PATHOGENIC VIRUSES; ROTAVIRUS; SEWAGE; IDENTIFICATION; CALIFORNIA; POLIOVIRUS;
D O I：
10.1504/IJEP.2009.026658
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A method for rapid detection of enteroviruses in water sample was established by using RT-PCR with universal primers with PV1, PV2, PV3 and CVB3 as the reference viruses. The conditions for simultaneous detection of different enteroviruses were experimentally determined and the detection limit of virus was found to be 38CCID(50). Virus concentration, RNA extraction and RT-PCR operation were the main factors affecting the sensitivity of virus detection. By applying the procedures to water samples with seeded PV1 viruses, the interference of impurities in practical waters on enteroviruses detection was investigated. The method was proved to be suitable for practical application.
引用
收藏
页码：151 / 165
页数：15
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