Multiplexing RT-PCR for the detection of multiple miRNA species in small samples

被引：130
|
作者：
Lao, KQ [1 ]
Xu, NL [1 ]
Yeung, V [1 ]
Chen, CF [1 ]
Livak, KJ [1 ]
Straus, NA [1 ]
机构：
[1] Appl Biosyst Inc, Foster City, CA 94404 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 343卷 / 01期
关键词：
multiplexed RT-PCR; miRNA; miRNA profiling; miRNA in small samples; multiple primer reverse transcription;
D O I：
10.1016/j.bbrc.2006.02.106
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs are short (similar to 22 nucleotides), non-coding RNAs that play critical roles in gene regulation and may be used as rapid precise diagnostic indicators of early stages of cancer. The small size of these RNAs makes detection of multiple microRNA species in very small samples problematic. Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：85 / 89
页数：5
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