Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation

被引：18

作者：

Kanamori, Takashi ^{[1
]}

Takamura, Akihiro ^{[1
]}

Tago, Nobuhiro ^{[1
]}

Masaki, Yoshiaki ^{[1
]}

Ohkubo, Akihiro ^{[1
]}

Sekine, Mitsuo ^{[1
]}

Seio, Kohji ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Life Sci, Midori Ku, 4259 Nagatsuta Cho, Yokohama, Kanagawa 2268501, Japan

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 05期

关键词：

PEPTIDE NUCLEIC-ACIDS; NUCLEOSIDE ANALOGS; MOLECULAR BEACONS; GFP CHROMOPHORE; DNA LABELS; BASE; PROBES; 2-AMINOPURINE; RECOGNITION; OLIGONUCLEOTIDES;

D O I：

10.1039/c6ob01278g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Green fluorescent protein (GFP)-based molecular-rotor chromophores were attached to the 5-positions of deoxyuridines, and subsequently, incorporated into the middle positions of oligodeoxynucleotides. These oligonucleotides were designed to form triplex DNA in order to encapsulate the GFP chromophores, mimicking GFP structures. Upon triplex formation, the embedded GFP chromophores exhibited fluorescence enhancement, suggesting the potential application of these fluorescent probes for the detection of nucleic acids.

引用

页码：1190 / 1197

页数：8

共 50 条

[41] Structure and Mechanism of RNA Mimics of Green Fluorescent Protein
You, Mingxu
Jaffrey, Samie R.
ANNUAL REVIEW OF BIOPHYSICS, VOL 44, 2015, 44 : 187 - 206
[42] Solvatochromism of the green fluorescence protein chromophore and its derivatives
Dong, Jian
Solntsev, Kyril M.
Tolbert, Laren M.
Journal of the American Chemical Society, 2006, 128 (37): : 12038 - 12039
[43] Solvatochromism of the green fluorescence protein chromophore and its derivatives
Dong, Jian
Solntsev, Kyril M.
Tolbert, Laren M.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (37) : 12038 - 12039
[44] Fluorescence Response Profiling for Small Molecule Sensors Utilizing the Green Fluorescent Protein Chromophore and Its Derivatives
Lee, Jun-Seok
Badridge, Anthony
Peng, Suihan
Yang SiQiang
Kim, Yun Kyung
Tolbert, Laren M.
Chang, Young-Tae
ACS COMBINATORIAL SCIENCE, 2011, 13 (01) : 32 - 38
[45] Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions
Rasmusssen, Anne P.
Pedersen, Henrik B.
Andersen, Lars H.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2023, 25 (48) : 32868 - 32874
[46] Absorption and fluorescence spectra of the neutral and anionic green fluorescent protein chromophore: Franck-Condon simulation
Huang, Tsung-wei
Yang, Ling
Zhu, Chaoyuan
Lin, Sheng Hsien
CHEMICAL PHYSICS LETTERS, 2012, 541 : 110 - 116
[47] Electric-Field-Induced Changes in Absorption and Fluorescence of the Green Fluorescent Protein Chromophore in a PMMA Film
Nakabayashi, Takakazu
Hino, Kazuyuki
Ohta, Yuka
Ito, Sayuri
Nakano, Hirofumi
Ohta, Nobuhiro
JOURNAL OF PHYSICAL CHEMISTRY B, 2011, 115 (26): : 8622 - 8626
[48] Green fluorescent protein is a suitable reporter of tumor hypoxia despite an oxygen requirement for chromophore formation
Vordermark, D
Shibata, T
Brown, JM
NEOPLASIA, 2001, 3 (06): : 527 - 534
[49] The crystal structure of a colorless variant of green fluorescent protein supports a novel mechanism for chromophore formation
Rosenow, MA
Huffman, HA
Patel, HN
Wachter, RM
PROTEIN SCIENCE, 2004, 13 : 150 - 150
[50] Self-Restricted Green Fluorescent Protein Chromophore Analogues: Dramatic Emission Enhancement and Remarkable Solvatofluorochromism
Deng, Hongping
Yu, Chunyang
Gong, Lidong
Zhu, Xinyuan
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2016, 7 (15): : 2935 - 2944

← 1 2 3 4 5 →