Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation

被引：18

作者：

Kanamori, Takashi ^{[1
]}

Takamura, Akihiro ^{[1
]}

Tago, Nobuhiro ^{[1
]}

Masaki, Yoshiaki ^{[1
]}

Ohkubo, Akihiro ^{[1
]}

Sekine, Mitsuo ^{[1
]}

Seio, Kohji ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Life Sci, Midori Ku, 4259 Nagatsuta Cho, Yokohama, Kanagawa 2268501, Japan

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 05期

关键词：

PEPTIDE NUCLEIC-ACIDS; NUCLEOSIDE ANALOGS; MOLECULAR BEACONS; GFP CHROMOPHORE; DNA LABELS; BASE; PROBES; 2-AMINOPURINE; RECOGNITION; OLIGONUCLEOTIDES;

D O I：

10.1039/c6ob01278g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Green fluorescent protein (GFP)-based molecular-rotor chromophores were attached to the 5-positions of deoxyuridines, and subsequently, incorporated into the middle positions of oligodeoxynucleotides. These oligonucleotides were designed to form triplex DNA in order to encapsulate the GFP chromophores, mimicking GFP structures. Upon triplex formation, the embedded GFP chromophores exhibited fluorescence enhancement, suggesting the potential application of these fluorescent probes for the detection of nucleic acids.

引用

页码：1190 / 1197

页数：8

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