Enzyme immunoassay for the detection of trimethoprim in raw milk

被引：0
|
作者：
Albrecht, U
Hammer, P
Heeschen, W
机构：
来源：
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL | 1996年 / 51卷 / 09期
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中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Trimethoprim (TMP) is mostly used in combination with different sulfonamides for the treatment of bacterial infections. In milk a maximum residue limit (MRL) for TMP is fixed at 50 mu g/kg. An ELISA was developed for the determination of TMP in raw milk. Antibodies with a high specificity were raised in rabbits. The ELISA was carried out as an indirect competitive antibody-capture test with a detection limit of 12.5 mu g/kg. Quantification is possible up to 100 mu g/kg without sample dilution. The immunoassay described is suitable as a screening method for the detection of TMP at the MRL and fulfils the requirements of the EU-Regulation 281/96.
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页码：515 / 516
页数：2
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