Development of enzyme linked immunosorbent assay and lateral flow immunoassay for the rapid detection of dapsone in milk

被引:5
|
作者
Guo, Xin [1 ]
Lin, Lu [1 ]
Song, Shanshan [1 ]
Wu, Aihong [1 ]
Liu, Liqiang [1 ]
Kuang, Hua [1 ]
Xu, Chuanlai [1 ]
机构
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
关键词
IMMUNOCHROMATOGRAPHIC STRIP; MONOCLONAL-ANTIBODY; ELECTROCHEMICAL SENSOR; ELISA; SULFONAMIDES; VALIDATION; RESIDUES;
D O I
10.1039/d1nj03247j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The excessive use of dapsone (DDS) in food animals could result in its accumulation in humans through the food chain, which in turn endangers human health. A sensitive monoclonal antibody (mAb) against DDS with a half inhibitory concentration (IC50) of 0.586 ng mL(-1) was prepared in this study. Furthermore, a lateral flow immunoassay (LFIA) was established for the detection of DDS based on the anti-DDS mAb. Under optimized conditions, the developed LFIA had outstanding sensitivity with a visual limit of detection (vLOD) and a cut-off value of 1 ng mL(-1) and 20 ng mL(-1) for DDS in milk, respectively, which could be used for semi-quantitative detection of DDS. In milk sample analysis, a red test line on the LFIA strip signified 20 ng mL(-1) DDS. In spiked samples and the recovery test, the results of the LFIA were in agreement with those of ic-ELISA, and the test using the LFIA strip could be completed within 15 minutes. Therefore, the established LFIA method in this study represents a reliable and compelling analysis for the rapid detection of DDS in milk.
引用
收藏
页码:19097 / 19104
页数:8
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