Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

被引：39

作者：

Bosse, Janine T. ^{[1
]}

Li, Yanwen ^{[1
]}

Angen, Oystein ^{[2
]}

Weinert, Lucy A. ^{[3
]}

Chaudhuri, Roy R. ^{[3
]}

Holden, Matthew T. ^{[4
]}

Williamson, Susanna M. ^{[5
]}

Maskell, Duncan J. ^{[4
]}

Tucker, Alexander W. ^{[4
]}

Wren, Brendan W. ^{[6
]}

Rycroft, Andrew N. ^{[7
]}

Langford, Paul R. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Med, Paediat Sect, London, England

[2] Norwegian Vet Inst, Oslo, Norway

[3] Univ Cambridge, Dept Vet Med, Cambridge, England

[4] Wellcome Trust Sanger Inst, Cambridge, England

[5] AHVLA, St Edmunds, Suffolk, England

[6] London Sch Hyg & Trop Med, Dept Pathogen Mol Biol, London WC1, England

[7] Univ London Royal Vet Coll, Dept Pathol & Pathogen Biol, London NW1 0TU, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

STRAINS; SEROTYPE-2; ELECTROPHORESIS; IDENTIFICATION;

D O I：

10.1128/JCM.00685-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

引用

页码：2380 / 2385

页数：6

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