Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

被引：39
|
作者：
Bosse, Janine T. [1 ]
Li, Yanwen [1 ]
Angen, Oystein [2 ]
Weinert, Lucy A. [3 ]
Chaudhuri, Roy R. [3 ]
Holden, Matthew T. [4 ]
Williamson, Susanna M. [5 ]
Maskell, Duncan J. [4 ]
Tucker, Alexander W. [4 ]
Wren, Brendan W. [6 ]
Rycroft, Andrew N. [7 ]
Langford, Paul R. [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Med, Paediat Sect, London, England
[2] Norwegian Vet Inst, Oslo, Norway
[3] Univ Cambridge, Dept Vet Med, Cambridge, England
[4] Wellcome Trust Sanger Inst, Cambridge, England
[5] AHVLA, St Edmunds, Suffolk, England
[6] London Sch Hyg & Trop Med, Dept Pathogen Mol Biol, London WC1, England
[7] Univ London Royal Vet Coll, Dept Pathol & Pathogen Biol, London NW1 0TU, England
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 07期
基金：
英国生物技术与生命科学研究理事会;
关键词：
STRAINS; SEROTYPE-2; ELECTROPHORESIS; IDENTIFICATION;
D O I：
10.1128/JCM.00685-14
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
引用
收藏
页码：2380 / 2385
页数：6
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