CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes

被引：2

作者：

Wang, Miao-xing ^{[1
]}

Itoh, Masanori ^{[1
]}

Li, Shimo ^{[1
]}

Hida, Yoko ^{[1
]}

Ohta, Kazunori ^{[1
]}

Hayakawa, Mild ^{[1
]}

Nishida, Emika ^{[1
]}

Ueda, Masashi ^{[1
]}

Islam, Saiful ^{[1
]}

Tana ^{[1
]}

Nakagawa, Toshiyuki ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Neurobiol, 1-1 Yanagido, Gifu 5011194, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 470卷 / 01期

关键词：

Apoptosis; RNA binding protein; L10a; Translation; Translocation; PROGRAMMED CELL-DEATH; CAENORHABDITIS-ELEGANS; C-ELEGANS; APOPTOSIS; ENCODES; INHIBITOR; INSIGHTS;

D O I：

10.1016/j.bbrc.2015.12.102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell death abnormal (ced)-3 and ced-4 genes regulate apoptosis to maintain tissue homeostasis in Caenorhabditis elegans. Apoptosome formation and CED-4 translocation drive CED-3 activation. However, the precise role of CED-4 translocation is not yet fully understood. In this study, using a combination of immunoprecipitation and reverse transcription-polymerase chain reaction methods in cells and a glutathione-S-transferase pull down assay in a cell-free system, we show that CED-4 binds ced-3 mRNA. In the presence of ced-3 mRNA, CED-4 protein is enriched in the microsomal fraction and interacts with ribosomal protein L10a in mammalian cells, increasing the levels of CED-3. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：48 / 53

页数：6

共 50 条

[41] Antiapoptotic Bcl-2 homolog CED-9 in Caenorhabditis elegans: Dynamics of BH3 and CED-4 binding regions and comparison with mammalian antiapoptotic Bcl-2 proteins
Modi, Vivek
Sankararamakrishnan, Ramasubbu
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2014, 82 (06) : 1035 - 1047
[42] The temporal relationship between protein phosphatase, ICE/CED-3 proteases, intracellular acidification, and DNA fragmentation in apoptosis
Wolf, CM
Reynolds, JE
Morana, SJ
Eastman, A
EXPERIMENTAL CELL RESEARCH, 1997, 230 (01) : 22 - 27
[43] The mRNA-Binding Protein HuR Is a Kinetically-Privileged Electrophile Sensor
Poganik, Jesse R.
Van Hall-Beauvais, Alexandra K.
Long, Marcus J. C.
Disare, Michael T.
Zhao, Yi
Aye, Yimon
HELVETICA CHIMICA ACTA, 2020, 103 (05)
[44] The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex
Shen, EC
Stage-Zimmermann, T
Chui, P
Silver, PA
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (31) : 23718 - 23724
[45] Post-transcriptional regulation of luteinizing hormone receptor mRNA in the ovary by a novel mRNA-binding protein
Nair, AK
Kash, JC
Peegel, H
Menon, KMJ
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (24) : 21468 - 21473
[46] The global regulator CsrA of Escherichia coli is a specific mRNA-binding protein
Liu, MY
Romeo, T
JOURNAL OF BACTERIOLOGY, 1997, 179 (14) : 4639 - 4642
[47] Overexpression of mRNA-binding protein CRD-BP in malignant melanomas
I Elcheva
R S Tarapore
N Bhatia
V S Spiegelman
Oncogene, 2008, 27 : 5069 - 5074
[48] Regulation of the mRNA-Binding Protein HuR by Posttranslational Modification: Spotlight on Phosphorylation
Eberhardt, Wolfgang
Doller, Anke
Pfeilschifter, Josef
CURRENT PROTEIN & PEPTIDE SCIENCE, 2012, 13 (04) : 380 - 390
[49] Glutamate dehydrogenase: an organelle-specific mRNA-binding protein - Reply
Lithgow, T
Gratzer, S
TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (08) : 290 - 290
[50] Overexpression of mRNA-binding protein CRD-BP in malignant melanomas
Elcheva, I.
Tarapore, R.
Bhatia, N.
Spiegelman, V. S.
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2008, 128 : S214 - S214

← 1 2 3 4 5 →