CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes

被引：2
|
作者：
Wang, Miao-xing [1 ]
Itoh, Masanori [1 ]
Li, Shimo [1 ]
Hida, Yoko [1 ]
Ohta, Kazunori [1 ]
Hayakawa, Mild [1 ]
Nishida, Emika [1 ]
Ueda, Masashi [1 ]
Islam, Saiful [1 ]
Tana [1 ]
Nakagawa, Toshiyuki [1 ]
机构：
[1] Gifu Univ, Grad Sch Med, Dept Neurobiol, 1-1 Yanagido, Gifu 5011194, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 470卷 / 01期
关键词：
Apoptosis; RNA binding protein; L10a; Translation; Translocation; PROGRAMMED CELL-DEATH; CAENORHABDITIS-ELEGANS; C-ELEGANS; APOPTOSIS; ENCODES; INHIBITOR; INSIGHTS;
D O I：
10.1016/j.bbrc.2015.12.102
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cell death abnormal (ced)-3 and ced-4 genes regulate apoptosis to maintain tissue homeostasis in Caenorhabditis elegans. Apoptosome formation and CED-4 translocation drive CED-3 activation. However, the precise role of CED-4 translocation is not yet fully understood. In this study, using a combination of immunoprecipitation and reverse transcription-polymerase chain reaction methods in cells and a glutathione-S-transferase pull down assay in a cell-free system, we show that CED-4 binds ced-3 mRNA. In the presence of ced-3 mRNA, CED-4 protein is enriched in the microsomal fraction and interacts with ribosomal protein L10a in mammalian cells, increasing the levels of CED-3. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：48 / 53
页数：6
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