CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes

被引：2

作者：

Wang, Miao-xing ^{[1
]}

Itoh, Masanori ^{[1
]}

Li, Shimo ^{[1
]}

Hida, Yoko ^{[1
]}

Ohta, Kazunori ^{[1
]}

Hayakawa, Mild ^{[1
]}

Nishida, Emika ^{[1
]}

Ueda, Masashi ^{[1
]}

Islam, Saiful ^{[1
]}

Tana ^{[1
]}

Nakagawa, Toshiyuki ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Neurobiol, 1-1 Yanagido, Gifu 5011194, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 470卷 / 01期

关键词：

Apoptosis; RNA binding protein; L10a; Translation; Translocation; PROGRAMMED CELL-DEATH; CAENORHABDITIS-ELEGANS; C-ELEGANS; APOPTOSIS; ENCODES; INHIBITOR; INSIGHTS;

D O I：

10.1016/j.bbrc.2015.12.102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell death abnormal (ced)-3 and ced-4 genes regulate apoptosis to maintain tissue homeostasis in Caenorhabditis elegans. Apoptosome formation and CED-4 translocation drive CED-3 activation. However, the precise role of CED-4 translocation is not yet fully understood. In this study, using a combination of immunoprecipitation and reverse transcription-polymerase chain reaction methods in cells and a glutathione-S-transferase pull down assay in a cell-free system, we show that CED-4 binds ced-3 mRNA. In the presence of ced-3 mRNA, CED-4 protein is enriched in the microsomal fraction and interacts with ribosomal protein L10a in mammalian cells, increasing the levels of CED-3. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：48 / 53

页数：6

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