To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the IBS-D model group (M); the syndrome differentiation acupuncture group (SD) and the traditional acupuncture group (T). IBS-D model rats were obtained using the CAS (chronic acute combining stress model) method. Comparative bioinformatics analysis of the proteomic data was analyzed using MaxQuant software, Perseus software, online tools DAVID, VENNY and STRING. Functional enrichment and network analyses revealed a close relationship between IBS-D and several biological processes including energy metabolism, muscular excitation/contraction, and both traditional acupuncture and syndrome differentiation acupuncture can reverse the impairments of normal energy metabolism. Moreover, the syndrome differentiation acupuncture can regulate the protein cluster relating inflammation, wound repair and cell protection against oxidative stress which is associated with acupuncture analgesic effect. Differentially expressed proteins Atp5a1 and Bpnt1 were selected as representative proteins and subjected to western blotting. In conclusion, our study provides further insight into the pathological and molecular mechanisms of IBS-D and acupuncture treatments, and serves as an experimental basis for clinical applications.
机构:
China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Beijing Univ Chinese Med, Beijing, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Guo, Jianbo
Xing, Xiaoxiao
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Beijing Univ Chinese Med, Beijing, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Xing, Xiaoxiao
Wu, Jiani
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China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Wu, Jiani
Zhang, Hui
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Henan Univ Chinese Med, Zhengzhou, Henan, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Zhang, Hui
Yun, Yongen
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Beijing Univ Chinese Med, Beijing, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Yun, Yongen
Qin, Zongshi
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Univ Hong Kong, Fac Med, Hong Kong, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
Qin, Zongshi
He, Qingyong
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China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R ChinaChina Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China
机构:
Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.ChinaShanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China
刘慧荣
杨允
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Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.ChinaShanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China
杨允
吴焕淦
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Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.ChinaShanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China
吴焕淦
肖元春
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Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.ChinaShanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China
机构:
Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
North Sichuan Med Coll, Nanchong, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Li, Yi-Lin
Yao, Cheng-Jiao
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Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
North Sichuan Med Coll, Affiliated Hosp, Dept Geriatr, Nanchong, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Yao, Cheng-Jiao
Lei, Rong
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North Sichuan Med Coll, Affiliated Hosp, Dept Hlth Management Ctr, Nanchong, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Lei, Rong
Xie, Fengjiao
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Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Xie, Fengjiao
Xiong, Qin
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Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Xiong, Qin
Luo, Li-Hong
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Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China
Luo, Li-Hong
Feng, Pei-Min
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Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Chengdu 610000, Sichuan, Peoples R China