TLC-UV and -VIS Densitometric Detection Method for Determination of Oxyphenonium Bromide and its Degradation Products in Tablets

被引：0
|
作者：
Hubicka, Urszula [1 ]
Krzek, Jan [1 ]
Szczyrbowska, Olga [1 ]
机构：
[1] Jagiellonian Univ, Dept Inorgan & Analyt Chem, Coll Med, PL-30688 Krakow, Poland
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2009年 / 32卷 / 09期
关键词：
Degradation products; Densitometry; Drug analysis; Oxyphenonium bromide; TLC; QUATERNARY AMMONIUM DRUGS; PAIR LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; REVERSED-PHASE; URINE; PLASMA;
D O I：
10.1080/10826070902854813
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A chromatographic-densitometric method was developed for determination of oxyhyphenonium bromide and its degradation products under UV/VIS spectra. Silica gel TLCF254 plates were used as stationary phase and butanol-1-glacial acetic acid-water (12.8:2:2.8 v/v/v) as mobile phase. Densitometric measurements were done at 225nm and 530nm. Degradation products -cyclohexyl--hydroxybenzeneacetic acid and N,N-diethyl-N-methylaminethanol were determined by 1HNMR method. The chromatographic-densitometric method is specific for studied compounds. Besides, it is shown that this method is very sensitive, precise, and has a broad range of linearity.
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页码：1196 / 1209
页数：14
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