A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays

被引：46

作者：

Chang-Yen, David A.

Myszka, David G.

Gale, Bruce K.

机构：

[1] Univ Utah, Utah State Ctr Excellence Biomed Microfluid, Salt Lake City, UT 84112 USA

[2] Univ Utah, Prot Interact Facil, HSC Core Labs, Salt Lake City, UT 84112 USA

[3] Univ Utah, Dept Mech Engn, Salt Lake City, UT 84112 USA

来源：

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS | 2006年 / 15卷 / 05期

基金：

美国国家科学基金会;

关键词：

microarray; microfluidic; polydimethyl siloxane (PDMS); spotter;

D O I：

10.1109/JMEMS.2006.880289

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A novel polydimethyl siloxane (PDMS) microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density. Microfluidic channels are used to address each spot region, and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samples compared to control and pipetted drops demonstrated a minimum of three-fold increase in dye surface density compared to pin-spotted dyes. Surface plasmon resonance (SPR) measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface concentration. The spotting system has been applied successfully to protein microarrays for SPR applications, in both a 12-spot linear and 48-spot two-dimensional (2-D) array format. This novel spotter system can be applied to the production of high-throughput arrays in the fields of genomics, proteomics, immunoassays, and fluorescence or luminescence assays.

引用

页码：1145 / 1151

页数：7

共 50 条

[21] Surface plasmon resonance imaging for affinity analysis of aptamer–protein interactions with PDMS microfluidic chips
Zhuangzhi Wang
Thomas Wilkop
Danke Xu
Yi Dong
Guangyu Ma
Quan Cheng
Analytical and Bioanalytical Chemistry, 2007, 389 : 819 - 825
[22] Easy-to-realise polyvinylsiloxane microfluidic connectors for PDMS chips
van den Driesche, S.
Pimentel, J. V.
Puchberger-Enengl, D.
Brandhoff, L.
Vellekoop, M. J.
EUROSENSORS 2015, 2015, 120 : 675 - 678
[23] Microfluidic Fabrication of a PDMS Microlens for Imaging Tunability
Zhang, Zhiguang
Chu, Fuqiang
Wang, Xin
Zhou, Xu
Xiong, Guirong
LANGMUIR, 2022, 38 (13) : 4059 - 4064
[24] Fast fabrication of PDMS microfluidic electrophoresis chip
State Key Lab of Transducer Technique, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China
J Fun Mater Dev, 2006, 4 (285-288):
[25] Study on surface properties of PDMS microfluidic chips treated with albumin
Schrott, Walter
Slouka, Zdenek
Cervenka, Petr
Ston, Jiri
Nebyla, Marek
Pribyl, Michal
Snita, Dalimil
BIOMICROFLUIDICS, 2009, 3 (04):
[26] Fabrication of microfluidic electrocontrolled chip in polydimethylsiloxane (PDMS)
Wang, M
Cui, DF
Wang, L
Chen, X
Zhao, Q
INTERNATIONAL JOURNAL OF NONLINEAR SCIENCES AND NUMERICAL SIMULATION, 2002, 3 (3-4) : 207 - 210
[27] Novel progress on microfluidic chips
Bulletin of the Chinese Academy of Sciences, 2008, (01) : 18 - 18
[28] Automatic fabrication system for plastic microfluidic chips
Wang, XD
Wang, LD
Liu, C
Ma, LQ
Luo, Y
PROCEEDINGS OF THE 2004 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE, 2004, : 331 - 336
[29] A biomolecule friendly photolithographic process for fabrication of protein microarrays on polymeric films coated on silicon chips
Petrou, Panagiota S.
Chatzichristidi, Margarita
Douvas, Antonios A.
Argitis, Panagiotis
Misiakos, Konstantinos
Kakabakos, Sotirios E.
BIOSENSORS & BIOELECTRONICS, 2007, 22 (9-10): : 1994 - 2002
[30] Feature characterization of microfabricated microfluidic chips by PDMS replication and CCD imaging
Dai, J
Guan, YX
Wang, SL
Wu, ZY
Fang, ZL
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 381 (04) : 839 - 843

← 1 2 3 4 5 →