Fast fabrication of PDMS microfluidic electrophoresis chip

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State Key Lab of Transducer Technique, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China [1 ]
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J Fun Mater Dev | 2006年 / 4卷 / 285-288期
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The figure of the microchannel was drawn with the Protel software. The mould was fabricated by the circuit board fabrication technique. The chip is composed of PDMS base slab and PDMS cover slab, and the microchannel whih 75μm in depth and l00μm in width is laid on the base slab sealed with the cover slab. The electrophoresis separations of the chips were investigated and the chips were fabricated by using the moulds coated with and without insulation lacquer. The performance of the chip is valuated by the determination of FITC fluorescein - labeled amino acids to a minimum detectable concentration of 0.8 × 10-11 mol/L, when the signal - to - noise - ratio ( SNR) S/N is 3.
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