Rapid, Sensitive and Simultaneous Detection of Two Wheat RNA Viruses Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA)
被引:7
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作者:
Chen, Zhiqing
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Chen, Zhiqing
[1
]
Zhang, Tianye
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Zhang, Tianye
[1
,2
]
Lei, Jiajia
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Lei, Jiajia
[1
]
Wang, Ziqiong
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Wang, Ziqiong
[1
,2
]
Liu, Peng
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Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Liu, Peng
[1
]
Zhong, Kaili
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Zhong, Kaili
[1
]
Chen, Jianping
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Chen, Jianping
[1
]
Liu, Jiaqian
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Liu, Jiaqian
[1
]
机构:
[1] Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
[2] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
In China, wheat yellow mosaic disease is mostly caused by wheat yellow mosaic virus (WYMV) and Chinese wheat mosaic virus (CWMV). If wheat is co-infected with these two viruses, it can cause severe yellow mosaic symptoms and yield losses. Early detection of viruses is crucial for preventing disease in the field. In this study, we optimized a sensitive, specific reverse transcription recombinase polymerase amplification (RT-RPA) detection method for two viruses, WYMV and CWMV. Two sets of primers were designed based on the capsid protein (CP)-encoding genes of the two viruses, and the reaction conditions were determined. The RT-RPA method, which amplified the target amplicon by a handheld reaction mixture for 20 min, was more sensitive than PCR-CP in the detection of WYMV. Finally, the RT-RPA method was performed on 110 randomly selected field samples, demonstrating its applicability to samples from different regions and specificity for co-infected samples. This study not only describes an improved method for detecting WYMV and CWMV using RT-RPA but also demonstrates the potential of this method, which could be applied under field conditions.
机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Center for Disease Control and Prevention of Shunyi DistrictState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Jia Jia
Yuchang Li
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Yuchang Li
Xiaoyan Wu
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Xiaoyan Wu
Sen Zhang
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Sen Zhang
Yi Hu
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Yi Hu
Jing Li
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Jing Li
Tao Jiang
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Graduate School,Anhui MedicalState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology
Tao Jiang
Xiaoping Kang
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机构:
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology