Rapid, Sensitive and Simultaneous Detection of Two Wheat RNA Viruses Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA)
被引:7
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作者:
Chen, Zhiqing
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Chen, Zhiqing
[1
]
Zhang, Tianye
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Zhang, Tianye
[1
,2
]
Lei, Jiajia
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Lei, Jiajia
[1
]
Wang, Ziqiong
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Wang, Ziqiong
[1
,2
]
Liu, Peng
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Liu, Peng
[1
]
Zhong, Kaili
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Zhong, Kaili
[1
]
Chen, Jianping
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Chen, Jianping
[1
]
Liu, Jiaqian
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机构:
Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R ChinaNingbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
Liu, Jiaqian
[1
]
机构:
[1] Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Peoples R China
[2] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
In China, wheat yellow mosaic disease is mostly caused by wheat yellow mosaic virus (WYMV) and Chinese wheat mosaic virus (CWMV). If wheat is co-infected with these two viruses, it can cause severe yellow mosaic symptoms and yield losses. Early detection of viruses is crucial for preventing disease in the field. In this study, we optimized a sensitive, specific reverse transcription recombinase polymerase amplification (RT-RPA) detection method for two viruses, WYMV and CWMV. Two sets of primers were designed based on the capsid protein (CP)-encoding genes of the two viruses, and the reaction conditions were determined. The RT-RPA method, which amplified the target amplicon by a handheld reaction mixture for 20 min, was more sensitive than PCR-CP in the detection of WYMV. Finally, the RT-RPA method was performed on 110 randomly selected field samples, demonstrating its applicability to samples from different regions and specificity for co-infected samples. This study not only describes an improved method for detecting WYMV and CWMV using RT-RPA but also demonstrates the potential of this method, which could be applied under field conditions.
机构:
Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R ChinaShanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
Zou, Xiaohua
Dong, Chao
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Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R ChinaShanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
Dong, Chao
Ni, Yiduo
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Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R ChinaShanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
Ni, Yiduo
Yuan, Shidong
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机构:
Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R ChinaShanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
Yuan, Shidong
Gao, Qing-Hua
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机构:
Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R ChinaShanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
机构:
Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Zang, Lianyi
Qiao, Ning
论文数: 0引用数: 0
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机构:
Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Weifang Univ Sci & Technol, Facil Hort Lab Univ Shandong, Shouguang 262700, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Qiao, Ning
Sun, Xiaohui
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Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Sun, Xiaohui
Zhang, Xianping
论文数: 0引用数: 0
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机构:
Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Zhang, Xianping
Zhao, Dan
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机构:
Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Zhao, Dan
Li, Jintang
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机构:
Weifang Univ Sci & Technol, Facil Hort Lab Univ Shandong, Shouguang 262700, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
Li, Jintang
Zhu, Xiaoping
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机构:
Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R ChinaShandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit Vegetable Qual & Eff, Tai An 271018, Shandong, Peoples R China
机构:
Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
China Minist Agr, Key Lab Anim Pathogen Biol, Northeastern Sci Inspect Stn, Harbin 150030, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
Ge, Junwei
Shi, Yunjia
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Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
Shi, Yunjia
Cui, Xingyang
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Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
Cui, Xingyang
Gu, Shanshan
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机构:
Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
Gu, Shanshan
Zhao, Lili
论文数: 0引用数: 0
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机构:
Chinese Acad Agr Sci, Harbin Vet Res Inst, Lab Anim & Comparat Med Unit, Harbin 150069, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
Zhao, Lili
Chen, Hongyan
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机构:
Chinese Acad Agr Sci, Harbin Vet Res Inst, Lab Anim & Comparat Med Unit, Harbin 150069, Heilongjiang, Peoples R ChinaNortheast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China
机构:
Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Jilin Univ, Coll Anim Med, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Cui, Huan
Zhang, Cheng
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Zhang, Cheng
Tu, Fei
论文数: 0引用数: 0
h-index: 0
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Tu, Fei
Zhao, Kui
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Jilin Univ, Coll Anim Med, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Zhao, Kui
Kong, Yunyi
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Kong, Yunyi
Pu, Jie
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Pu, Jie
Zhang, Lei
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Zhang, Lei
Chen, Zhaoliang
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h-index: 0
机构:
Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Chen, Zhaoliang
Sun, Yuanyuan
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h-index: 0
机构:
Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Sun, Yuanyuan
Wei, Yujie
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Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Wei, Yujie
Liang, Chuncai
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Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Liang, Chuncai
Liu, Juxiang
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Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Liu, Juxiang
Liu, Jun
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Liu, Jun
Guo, Zhendong
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Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R ChinaChinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
Guo, Zhendong
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