Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA

被引：55
|
作者：
Zainah, S. [1 ]
Wahab, A. H. Abdul [2 ]
Mariam, M. [2 ]
Fauziah, M. K. [1 ]
Khairul, A. H. [2 ]
Roslina, I. [1 ]
Sairulakhma, A. [1 ]
Kadimon, S. S. [2 ]
Jais, M. S. Mat [2 ]
Chua, K. B. [2 ]
机构：
[1] Inst Med Res, Infect Dis Res Ctr, Virol Unit, Kuala Lumpur 50588, Malaysia
[2] Minist Hlth, Natl Publ Hlth Lab, Sungai Buloh 47000, Selangor, Malaysia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2009年 / 155卷 / 02期
关键词：
Dengue; Rapid Antigen Detection; Diagnosis; Immunochromatography; NONSTRUCTURAL GLYCOPROTEIN NS1; BORNE ENCEPHALITIS-VIRUS; JAPANESE ENCEPHALITIS; HEMORRHAGIC-FEVER; PROTEIN NS1; CELLS; DIAGNOSIS; SECRETION; ANTIBODY; SERA;
D O I：
10.1016/j.jviromet.2008.10.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 160
页数：4
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