Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA

被引：55

作者：

Zainah, S. ^{[1
]}

Wahab, A. H. Abdul ^{[2
]}

Mariam, M. ^{[2
]}

Fauziah, M. K. ^{[1
]}

Khairul, A. H. ^{[2
]}

Roslina, I. ^{[1
]}

Sairulakhma, A. ^{[1
]}

Kadimon, S. S. ^{[2
]}

Jais, M. S. Mat ^{[2
]}

Chua, K. B. ^{[2
]}

机构：

[1] Inst Med Res, Infect Dis Res Ctr, Virol Unit, Kuala Lumpur 50588, Malaysia

[2] Minist Hlth, Natl Publ Hlth Lab, Sungai Buloh 47000, Selangor, Malaysia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 155卷 / 02期

关键词：

Dengue; Rapid Antigen Detection; Diagnosis; Immunochromatography; NONSTRUCTURAL GLYCOPROTEIN NS1; BORNE ENCEPHALITIS-VIRUS; JAPANESE ENCEPHALITIS; HEMORRHAGIC-FEVER; PROTEIN NS1; CELLS; DIAGNOSIS; SECRETION; ANTIBODY; SERA;

D O I：

10.1016/j.jviromet.2008.10.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：157 / 160

页数：4

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