Finding better protein engineering strategies

被引：164
|
作者：
Kazlauskas, Romas J. [1 ,2 ]
Bornscheuer, Uwe T. [3 ]
机构：
[1] Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
[2] Univ Minnesota, Dept Biochem Mol Biol & Biophys, St Paul, MN 55108 USA
[3] Ernst Moritz Arndt Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, Greifswald, Germany
来源：
NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 08期
关键词：
DIRECTED EVOLUTION; SATURATION MUTAGENESIS; ENZYME EVOLUTION; DRIFT;
D O I：
10.1038/nchembio0809-526
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein improvement strategies today involve widely varying combinations of rational design with random mutagenesis and screening. To make further progress-defined as making subsequent protein engineering problems easier to solve-protein engineers must critically compare these strategies and eliminate less effective ones.
引用
收藏
页码：526 / 529
页数：4
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