3-D stimulated emission depletion microscopy with programmable aberration correction

被引：70

作者：

Lenz, Martin O. ^{[1
]}

Sinclair, Hugo G. ^{[1
]}

Savell, Alexander ^{[2
]}

Clegg, James H. ^{[1
]}

Brown, Alice C. N. ^{[3
]}

Davis, Daniel M. ^{[3
]}

Dunsby, Chris ^{[1
,4
]}

Neil, Mark A. A. ^{[1
]}

French, Paul M. W. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Photon Grp, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Inst Chem Biol, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Div Cell & Mol Biol, London SW7 2AZ, England

[4] Univ London Imperial Coll Sci Technol & Med, Ctr Histopathol, London W12 0NN, England

来源：

JOURNAL OF BIOPHOTONICS | 2014年 / 7卷 / 1-2期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

fluorescence microscopy; confocal microscopy; STED microscopy; nanoscopy; super-resolution; immune synapse; STRUCTURED-ILLUMINATION MICROSCOPY; FLUORESCENCE MICROSCOPY; STED MICROSCOPY; POLARIZATION CONTROL; RESOLUTION LIMIT; LIGHT; NANOSCOPY; BREAKING; REVEALS; BARRIER;

D O I：

10.1002/jbio.201300041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：29 / 36

页数：8

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