In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies

被引：0
|
作者：
Härtig, W
Varga, C
Kacza, J
Grosche, J
Seeger, J
Luiten, PGM
Brauer, K
Harkany, T
机构：
[1] Univ Leipzig, Paul Flechsig Inst Brain Res, D-04109 Leipzig, Germany
[2] Univ Leipzig, Dept Anat Histol & Embryol, D-04103 Leipzig, Germany
[3] Univ Groningen, Dept Mol Neurobiol, NL-9750 AA Haren, Netherlands
[4] Univ Szeged, Fac Med, Dept Med Chem, Szeged, Hungary
来源：
NEUROREPORT | 2002年 / 13卷 / 11期
关键词：
choline acetyltransferase; cholinergic system; low-affinity neurotrophin receptor; oxygen-enriched photoconversion;
D O I：
暂无
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Cholinergic basal forebrain neurons (CBFN) expressing the low-affinity neurotrophin receptor p75 (p75(NTR)) were previously selectively labeled in vivo with carbocyanine 3 (Cy3)-tagged anti-p75(NTR), but the applied 192IgG-conjugates recognized p75(NTR) only in rat The antibody ME 20.4 raised against human p75(NTR) had been shown to cross-react with the receptor in monkey, raccoon, sheep, cat, dog, pig and rabbit. Hence, for in vivo labeling of rabbit CBFN in the present study, ME 20.4 was fluorochromated with Cy3-N-hydroxysuccinimide ester and purified Cy3-ME 20.4 was injected intracerebroventricularly. Two days post-injection, clusters of Cy3-ME 20.4 were found in CBFN displaying choline acetyltransferase-immunoreactivity. Following photoconversion, electron microscopy revealed fluorochromated antibodies in secondary lysosomes. In conclusion, Cy3-ME 20.4 might become an appropriate marker for CBFN in live and fixed tissues of various mammalian species.
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收藏
页码：1395 / 1398
页数：4
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